Signal transducer and activator of transcription 3 (STAT3) homologue in turbot (Scophthalmus maximus): molecular characterization and expression analysis.
Signal transducer and activator of transcription 3 (STAT3) homologue in turbot (Scophthalmus maximus): molecular characterization and expression analysis.
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DOI：
10.1016/j.fsi.2010.10.017
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发表时间：
2011
影响因子：
4.7
通讯作者：
Na Wang;Changgeng Yang;Zhongzhi Sun;Xianli Wang;Song‐lin Chen
中科院分区：
文献类型：
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作者：
Na Wang;Changgeng Yang;Zhongzhi Sun;Xianli Wang;Song‐lin Chen
信号转导子和转录激活子3（STAT 3）在多种细胞因子诱导的多种生物学过程中起重要的介导作用。到目前为止，鱼类STAT 3的信息很少。本研究首次克隆了大菱鲆STAT 3基因，并对其进行了序列分析。大菱鲆STAT 3全长cDNA由2355个核苷酸组成，编码784个氨基酸，具有STAT_int、STAT_alpha、STAT_bind和SH 2结构域4个保守结构域。系统进化树显示大菱鲆STAT 3与鳜鱼STAT 3的亲缘关系最近。在酵母中的自激活实验证明，大菱鲆STAT 3是一个强转录因子。定量RT-PCR结果表明，Stat 3 mRNA在肝脏中的表达量最高，在肝脏中的表达量最高。进一步的表达谱分析表明，Stat 3在鳗弧菌感染的大菱鲆肝脏、脾脏、肾脏和LCDV感染的大菱鲆肝脏中的表达量均增加。同时，STAT 3靶基因之一hepcidin在感染两种病原体的鱼肝脏中也表达上调。这些结果提示Stat 3可能作为转录因子参与了大菱鲆的免疫防御过程。
Signal transducer and activator of transcription 3 (STAT3) acts as an important mediator in multiple biological processes induced by different cytokines. So far, little information is available in fish STAT3. In this study, turbot (Scophthalmus maximus) STAT3 gene was cloned and characterized for the first time. The turbot STAT3 full-length cDNA consists of 2355 nucleotides encoding a polypeptide of 784 amino acids with four conserved domains including STAT_int, STAT_alpha, STAT_bind and SH2 domain. The phylogenetic tree showed that turbot STAT3 shared the closest relationship with mandarin fish (Siniperca chuatsi) STAT3. The autoactivation experiment in yeast proved that turbot STAT3 was a strong transcription factor. The quantitative RT-PCR experiment indicated that Stat3 mRNA was expressed in widespread tissues with the highest expression levels in the liver. And the further expression patterns analysis revealed that turbot Stat3 expression levels were increased in liver, spleen, kidney of fish infected with Vibrio anguillarum and liver of fish infected with LCDV. Meantime, hepcidin, one of STAT3 target gene, was also up-regulated in liver of fish infected with two pathogens. These results suggested that turbot Stat3 may involved in the immune defense process as a transcription factor.