A 2ND GENE FOR THE AFRICAN-GREEN MONKEY POLIOVIRUS RECEPTOR THAT HAS NO PUTATIVE N-GLYCOSYLATION SITE IN THE FUNCTIONAL N-TERMINAL IMMUNOGLOBULIN-LIKE DOMAIN
A 2ND GENE FOR THE AFRICAN-GREEN MONKEY POLIOVIRUS RECEPTOR THAT HAS NO PUTATIVE N-GLYCOSYLATION SITE IN THE FUNCTIONAL N-TERMINAL IMMUNOGLOBULIN-LIKE DOMAIN
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DOI：
10.1128/jvi.66.12.7059-7066.1992
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发表时间：
1992-12-01
影响因子：
5.4
通讯作者：
NOMOTO, A
中科院分区：
文献类型：
--
作者：
KOIKE, S;ISE, I;NOMOTO, A
以人脊髓灰质炎病毒受体（PVR）的cDNA为探针，从非洲绿色猴肾细胞系制备的cDNA文库中分离出猴PVR同源物的两种cDNA克隆。任何一种类型的cDNA克隆使小鼠L细胞允许脊髓灰质炎病毒感染。从这些cDNA序列推导的氨基酸序列与人PVR的同源性分别为90.2%和86.4%。发现这两种猴PVR在基因组的两个不同位点中编码。进化分析表明，猴基因组中PVR基因的重复发生在人类和猴子之间的物种分化之后。第二个猴PVR的NH 2-末端免疫球蛋白样结构域（结构域1）缺乏推定的N-糖基化位点，介导脊髓灰质炎病毒感染。此外，在结构域1中没有N-糖基化位点的人PVR突变体也促进病毒感染。这些结果表明，猴受体的结构域1也含有脊髓灰质炎病毒的结合位点，并且可能连接到人PVR的该结构域的糖部分对于病毒-受体相互作用是不稳定的。
Using cDNA of the human poliovirus receptor (PVR) as a probe, two types of cDNA clones of the monkey homologs were isolated from a cDNA library prepared from an African green monkey kidney cell line. Either type of cDNA clone rendered mouse L cells permissive for poliovirus infection. Homologies of the amino acid sequences deduced from these cDNA sequences with that of human PVR were 90.2 and 86.4%, respectively. These two monkey PVRs were found to be encoded in two different loci of the genome. Evolutionary analysis suggested that duplication of the PVR gene in the monkey genome had occurred after the species differentiation between humans and monkeys. The NH2-terminal immunoglobulin-like domain, domain 1, of the second monkey PVR, which lacks a putative N-glycosylation site, mediated poliovirus infection. In addition, a human PVR mutant without N-glycosylation sites in domain 1 also promoted viral infection. These results suggest that domain 1 of the monkey receptor also harbors the binding site for poliovirus and that sugar moieties possibly attached to this domain of human PVR are dispensable for the virus-receptor interaction.