C/EBPbeta is required for 'emergency' granulopoiesis.
C/EBPbeta is required for 'emergency' granulopoiesis.
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DOI：
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发表时间：
2006
影响因子：
30.5
通讯作者：
H. Hirai;Pu Zhang;Tajhal Dayaram;C. Hetherington;S. Mizuno;J. Imanishi;K. Akashi;D. Tenen
中科院分区：
文献类型：
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作者：
H. Hirai;Pu Zhang;Tajhal Dayaram;C. Hetherington;S. Mizuno;J. Imanishi;K. Akashi;D. Tenen
在“紧急”情况下，如感染，宿主防御需要快速动员骨髓粒细胞祖细胞。“稳态”粒细胞生成完全依赖于C/EBPalpha转录因子，但应急粒细胞生成的转录机制仍不清楚。在这里，我们表明，大量的粒细胞产生的C/EBPa缺陷的祖细胞后，在体内细胞因子刺激。细胞因子治疗或真菌感染诱导的上调C/EB β，但不C/EB α或C/EB肽转录粒细胞祖细胞，和C/EB β缺陷的祖细胞显示减少紧急诱导的粒细胞生成在体外和体内。C/EBP β对增殖的抑制作用不如C/EBP α严重。这些数据表明C/EBP β在紧急粒细胞生成中的关键功能，其需要粒细胞前体的分化和增殖。
During 'emergency' situations such as infections, host defense requires rapid mobilization of bone marrow granulocyte progenitors. 'Steady-state' granulopoiesis is absolutely dependent on the C/EBPalpha transcription factor, but the transcriptional mechanisms underlying emergency granulopoiesis remain unclear. Here we show that large numbers of granulocytes were generated from C/EBPalpha-deficient progenitors after cytokine stimulation in vivo. Cytokine treatment or fungal infection induced upregulation of C/EBPbeta but not C/EBPalpha or C/EBPepsilon transcripts in granulocyte progenitors, and C/EBPbeta-deficient progenitors showed decreased emergency-induced granulopoiesis in vitro and in vivo. C/EBPbeta inhibited proliferation less severely than did C/EBPalpha. These data suggest a critical function for C/EBPbeta in emergency granulopoiesis, which demands both differentiation and proliferation of granulocyte precursors.