Isolation of a new type of human papillomavirus (HPV52b) with a transforming activity from cervical cancer tissue.
Isolation of a new type of human papillomavirus (HPV52b) with a transforming activity from cervical cancer tissue.
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从宫颈癌组织中分离出具有转化活性的新型人乳头瘤病毒 (HPV52b)。
作者：
H. Yajima;T. Noda;E. Villiers;A. Yajima;Kaiichiro Yamamoto;Kiichi Noda;Yoshiaki Ito
大量证据表明人乳头瘤病毒（HPV）是宫颈癌的病原体。我们检测并克隆了一个未知的HPV基因组的细胞DNA提取的手术标本的日本宫颈癌患者。杂交研究表明，该HPV是迄今已鉴定的51种HPV中与HPV33同源性最高的一种。最近发现了一种新的HPV，由W.兰开斯特命名为HPV52，与HPV33（W.兰开斯特等人，未公布的数据）。我们的HPV被发现是同源的，但不相同的HPV52，因此被指定为HPV52b。将其导入NIH3T3细胞，发现HPV52b DNA与HPV16 DNA一样具有促生长活性。这种新鉴定的HPV52b DNA作为游离体存在于迄今为止检查的两个标本中的两个宫颈癌细胞中，并且未检测到整合拷贝。在日本，15例标本中有3例（20%）存在HPV52b。此外，在其他三份标本中检测到三种与HPV52b同源的额外未知HPV序列。结果表明，这组HPV在日本可能是普遍存在的，并参与宫颈癌的致癌作用。
Substantial evidence has implicated human papillomaviruses (HPVs) as etiological agents of human cervical cancer. We detected and cloned an unidentified HPV genome in cellular DNA extracted from a surgical specimen of a Japanese patient with cervical cancer. Hybridization studies suggested that this HPV was the most homologous to HPV33 among more than 51 types of HPV ever identified. A recently identified new HPV, isolated by W. Lancaster and designated HPV52, is also most homologous to HPV33 (W. Lancaster et al., unpublished data). Our HPV was found to be homologous but not identical to HPV52 and hence was designated HPV52b. By introducing it into NIH3T3 cells, we found that HPV52b DNA had growth-stimulating activity as HPV16 DNA. This newly identified HPV52b DNA was present as episomes in cervical cancer cells of two out of two specimens so far examined and the integrated copies were not detected. HPV52b was present in three out of 15 (20%) specimens in Japan. In addition, the presence of three additional unidentified HPV sequences homologous to HPV52b was detected in three other specimens. The results suggest that this group of HPV may be prevalent and involved in carcinogenesis of cervical cancer in Japan.