The aim of the measurement of Epstein-Barr virus DNA in hydroa vacciniforme and hypersensitivity to mosquito bites.
The aim of the measurement of Epstein-Barr virus DNA in hydroa vacciniforme and hypersensitivity to mosquito bites.
复制标题
测量痘痘水母中的 Epstein-Barr 病毒 DNA 和对蚊虫叮咬的过敏反应的目的。
DOI：
10.1002/jmv.25811
复制
发表时间：
2020
影响因子：
12.7
通讯作者：
Morizane S.
中科院分区：
文献类型：
--
作者：
Miyake T;Iwatsuki K;Hirai Y;Yamamoto T;Hamada T;Fujii K;Imamura H;Morizane S.
在移植后淋巴组织增生性疾病和慢性活动性EBV感染中，血液中的EB巴尔病毒（EBV）DNA载量增加。本研究分析了种痘样水肿（HV）和蚊虫叮咬致敏（HMB）患者外周血单个核细胞（PBMC）和血浆中EBV DNA载量，以了解EBV DNA载量的临床意义。所有30例患者均显示PBMC中的高DNA载量超过临界水平。在16份血浆样本中，从噬血细胞性淋巴组织细胞增多症（HLH）患者中获得的两份样本中的浓度极高。血浆中无细胞DNA的量与血清乳酸脱氢酶水平相关，与血小板计数呈负相关。这些结果表明，PBMC中的EBV DNA载量可以提供HV和HMB的诊断指标之一，血浆中无细胞EBV DNA的显著升高可能与HLH中观察到的细胞溶解有关。
Epstein‐Barr virus (EBV) DNA load in the blood increases in posttransplant lymphoproliferative disorders and chronic active EBV infection. In this report, we analyzed the EBV DNA load in the peripheral blood mononuclear cells (PBMCs) and plasma of patients with hydroa vacciniforme (HV) and/or hypersensitivity to mosquito bites (HMB) to understand the clinical significance of EBV DNA load. All 30 patients showed high DNA loads in the PBMCs over the cut‐off level. Of 16 plasma samples, extremely high in two samples obtained from patients with hemophagocytic lymphohistiocytosis (HLH). The amount of cell‐free DNA in plasma was correlated to the serum levels of lactate dehydrogenase and inversely correlated to platelet counts. These results indicate that the EBV DNA load in PBMCs can provide one of the diagnostic indicators for HV and HMB and marked elevation of cell‐free EBV DNA in plasma might be related to cytolysis such as that observed in HLH.