The bacteriophage f1 morphogenetic signal and the gene V protein/phage single-stranded DNA complex.
The bacteriophage f1 morphogenetic signal and the gene V protein/phage single-stranded DNA complex.
复制标题
噬菌体 f1 形态发生信号和基因 V 蛋白/噬菌体单链 DNA 复合物。
DOI：
10.1016/0042-6822(84)90399-4
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发表时间：
1984
期刊：
影响因子：
3.7
通讯作者：
Webster,RE
中科院分区：
文献类型：
--
作者：
Grant,RA;Webster,RE
噬菌体 f1 基因组靠近基因 IV/基因间区域 (IG) 连接的区域先前已被证明包含将单链 (SS) DNA 有效包装成噬菌体颗粒所需的序列（f1“形态发生信号”）[G. P. Dotto、V. Enea 和 N. D. Zinder (1981)Virology114, 463–473]。对 f1 噬菌体/pBR322 嵌合质粒转化的噬菌体感染细菌的 DNA 含量进行了研究。 Dottoet al. (1981) 构建的嵌合质粒包含 DNA 复制的 f1 起点，在某些情况下还包含“形态发生信号”。在携带任何一种嵌合质粒的噬菌体感染细菌中检测到嵌合质粒 SS DNA，并且发现该 SS DNA 的大部分与噬菌体基因 V 蛋白复合。因此，“形态发生信号”对于基因V蛋白/f1 SS DNA复合物的形成不是必需的，而是必须在丝状噬菌体形态发生的后期发挥作用。
The region of the bacteriophage f1 genome near the gene IV/intergenic region (IG) junction previously was shown to contain a sequence necessary for efficient packaging of single-stranded (SS) DNA into phage particles (the f1 “morphogenetic signal”) [G. P. Dotto, V. Enea, and N. D. Zinder (1981)Virology114, 463–473]. The DNA content of f1 phage/pBR322 chimeric plasmid-transformed, phage-infected bacteria has been investigated. The chimeric plasmids, constructed by Dottoet al.(1981), contained the f1 origins of DNA replication and, in some cases, also contained the “morphogenetic signal.” Chimeric plasmid SS DNA was detected in phage-infected bacteria harboring any one of these chimeric plasmids, and the majority of this SS DNA was found complexed to the phage gene V protein. Therefore, the “morphogenetic signal” is not required for the formation of the gene V protein/f1 SS DNA complex but instead must function at a later stage of filamentous phage morphogenesis.