Tumor necrosis factor alpha has an early protective effect on retinal ganglion cells after optic nerve crush.
Tumor necrosis factor alpha has an early protective effect on retinal ganglion cells after optic nerve crush.
复制标题
DOI：
10.1186/s12974-014-0194-3
复制
发表时间：
2014-11-19
影响因子：
9.3
通讯作者：
Nickells RW
中科院分区：
文献类型：
--
作者：
Mac Nair CE;Fernandes KA;Schlamp CL;Libby RT;Nickells RW
Glaucoma is an optic neuropathy that is characterized by the loss of retinal ganglion cells (RGCs) initiated by damage to axons in the optic nerve. The degeneration and death of RGCs has been thought to occur in two waves. The first is axogenic, caused by direct insult to the axon. The second is somatic, and is thought to be caused by the production of inflammatory cytokines from the activated retinal innate immune cells. One of the cytokines consistently linked to glaucoma and RGC damage has been TNFα. Despite strong evidence implicating this protein in neurodegeneration, a direct injection of TNFα does not mimic the rapid loss of RGCs observed after acute optic nerve trauma or exposure to excitotoxins. This suggests that our understanding of TNFα signaling is incomplete. RGC death was induced by optic nerve crush in mice. The role of TNFα in this process was examined by quantitative PCR of Tnfα gene expression, and quantification of cell loss in Tnfα−/− mice or in wild-type animals receiving an intraocular injection of exongenous TNFα either before or after crush. Signaling pathways downstream of TNFα were examined by immunolabeling for JUN protein accumulation or activation of EGFP expression in NFκB reporter mice. Optic nerve crush caused a modest increase in Tnfα gene expression, with kinetics similar to the activation of both macroglia and microglia. A pre-injection of TNFα attenuated ganglion cell loss after crush, while ganglion cell loss was more severe in Tnfα−/− mice. Conversely, over the long term, a single exposure to TNFα induced extrinsic apoptosis in RGCs. Müller cells responded to exogenous TNFα by accumulating JUN and activating NFκB. Early after optic nerve crush, TNFα appears to have a protective role for RGCs, which may be mediated through Müller cells.
登录
查看更多内容
影响因子：
6.1
作者：
Fernandes, Kimberly A.;Harder, Jeffrey M.;Fornarola, Laura B.;Freeman, Robert S.;Clark, Abbot F.;Pang, Iok-Hou;John, Simon W. M.;Libby, Richard T.
通讯作者：
Libby, Richard T.
影响因子：
3.4
作者：
Fernandes, Kimberly A.;Harder, Jeffrey M.;Kim, Jessica;Libby, Richard T.
通讯作者：
Libby, Richard T.
影响因子：
3.3
作者：
Baptiste, DC;Powell, KJ;Kelly, MEM
通讯作者：
Kelly, MEM
影响因子：
4.8
作者：
Dhandapani, KM;Hadman, M;Brann, DW
通讯作者：
Brann, DW
影响因子：
4.4
作者：
Janssen, Katherine T.;Mac Nair, Caitlin E.;Nickells, Robert W.
通讯作者：
Nickells, Robert W.