Simultaneous monitoring of the expression of multiple genes inside of single breast carcinoma cells
Simultaneous monitoring of the expression of multiple genes inside of single breast carcinoma cells
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DOI：
10.1021/ac050881y
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发表时间：
2005-08-01
影响因子：
7.4
通讯作者：
Tan, WH
中科院分区：
文献类型：
--
作者：
Medley, CD;Drake, TJ;Tan, WH
监测基因表达是多种医学、生物学和生物技术应用研究的核心。目前，还没有一种方法能够在单个活细胞内真正监测多个基因的表达，并使该细胞的基因表达谱能够与另一个单个活细胞直接进行比较。通过将带有不同荧光团的多个分子信标微量注射到单个乳腺癌细胞内，并使用先进的荧光显微镜进行监测，可以在单个活细胞内同时监测多个基因的表达。以比率分析作为测量的基础，可以对不同细胞之间的不同基因表达水平进行比较。这不仅可以区分多个单个细胞之间的单个mRNA表达水平，还可以在单细胞水平上进行mRNA表达趋势分析。
Monitoring gene expression is at the center of research for a wide variety of medical, biological, and biotechnological applications. Currently no method exists for true multiple gene expression monitoring inside of a single living cell that allows for the gene expression profile of the cell to be directly compared with another single living cell. By microinjecting multiple molecular beacons with different fluorophores inside of single breast carcinoma cells and monitoring with advanced fluorescent microscopy, the expression of multiple genes can be simultaneously monitored inside single living cells. Using ratiometric analysis as a basis for the measurements allows the different gene expression levels to be compared from cell to cell. Not only does this allow differentiation of individual mRNA expression levels between multiple single cells but it also allows for mRNA expression trend analysis at the single cell level.