RTM3, Which Controls Long-Distance Movement of Potyviruses, Is a Member of a New Plant Gene Family Encoding a Meprin and TRAF Homology Domain-Containing Protein
RTM3, Which Controls Long-Distance Movement of Potyviruses, Is a Member of a New Plant Gene Family Encoding a Meprin and TRAF Homology Domain-Containing Protein
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DOI：
10.1104/pp.110.155754
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发表时间：
2010-09-01
期刊：
影响因子：
7.4
通讯作者：
Revers, Frederic
中科院分区：
文献类型：
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作者：
Cosson, Patrick;Sofer, Luc;Revers, Frederic
拟南芥（Arabidopsis thaliana）中几种马铃薯Y病毒长距离移动的限制由至少三个显性的限制TEV移动（RTM）基因控制，分别命名为RTM1、RTM2和RTM3。RTM1编码一种属于木菠萝凝集素家族的蛋白质，RTM2编码一种与小分子热激蛋白具有相似性的蛋白质。在本文中，我们描述了RTM3的定位克隆，它编码一种属于一个包含29个成员的未描述蛋白质家族的蛋白质，该蛋白质在其氨基末端区域具有一个Meprin和TRAF同源（MATH）结构域，在其羧基末端具有一个卷曲螺旋结构域。参与RTM抗性系统是在植物中通过实验确定的这个新基因家族成员的第一个生物学功能。我们的分析表明，卷曲螺旋结构域不仅在RTM3同源的含MATH蛋白质之间高度保守，而且在缺乏MATH结构域的蛋白质中也是如此。拟南芥基因组中RTM3同源物的簇状组织表明，复制事件在塑造这个基因家族的进化历史中起作用，包括一个或另一个结构域缺失或复制的可能性。蛋白质 - 蛋白质相互作用实验揭示了RTM3的自身相互作用以及RTM1 - RTM3的相互作用。然而，没有检测到涉及RTM2或先前已表明是克服RTM抗性所必需的决定因素的马铃薯Y病毒外壳蛋白的相互作用。综上所述，这些观察结果强烈表明，RTM蛋白质可能在抗性机制中形成一个多蛋白复合物，以阻止马铃薯Y病毒的长距离移动。
Restriction of long-distance movement of several potyviruses in Arabidopsis (Arabidopsis thaliana) is controlled by at least three dominant restricted TEV movement (RTM) genes, named RTM1, RTM2, and RTM3. RTM1 encodes a protein belonging to the jacalin family, and RTM2 encodes a protein that has similarities to small heat shock proteins. In this article, we describe the positional cloning of RTM3, which encodes a protein belonging to an undescribed protein family of 29 members that has a meprin and TRAF homology (MATH) domain in its amino-terminal region and a coiled-coil domain at its carboxy-terminal end. Involvement in the RTM resistance system is the first biological function experimentally identified for a member of this new gene family in plants. Our analyses showed that the coiled-coil domain is not only highly conserved between RTM3-homologous MATH-containing proteins but also in proteins lacking a MATH domain. The cluster organization of the RTM3 homologs in the Arabidopsis genome suggests the role of duplication events in shaping the evolutionary history of this gene family, including the possibility of deletion or duplication of one or the other domain. Protein-protein interaction experiments revealed RTM3 self-interaction as well as an RTM1-RTM3 interaction. However, no interaction has been detected involving RTM2 or the potyviral coat protein previously shown to be the determinant necessary to overcome the RTM resistance. Taken together, these observations strongly suggest the RTM proteins might form a multiprotein complex in the resistance mechanism to block the long-distance movement of potyviruses.