Transcriptional precision and accuracy in development: from measurements to models and mechanisms.
Transcriptional precision and accuracy in development: from measurements to models and mechanisms.
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DOI：
10.1242/dev.146563
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发表时间：
2017-11-01
期刊：
影响因子：
--
通讯作者：
DePace AH
中科院分区：
文献类型：
--
作者：
Bentovim L;Harden TT;DePace AH
在发育过程中，基因在特定的时间、位置和水平转录。近年来，定量工具的出现大大提高了我们在体内以高时空分辨率测量转录的能力。在这里，我们强调了最近的研究，使用这些工具来表征转录在发展过程中，并讨论了机制，有助于精确度和准确性的时间，位置和转录水平。我们试图解开物理学家和生物学家如何使用术语“精度”，以促进使用共同语言的相互作用的差异。我们还强调了选定的例子，其中数学建模与实验方法的耦合提供了重要的机制的见解，并呼吁更广泛地使用数学建模，以利用丰富的定量数据，并推进我们对动物转录的理解。总结：这篇综述强调了高分辨率的定量工具和理论模型是如何形成我们目前对果蝇胚胎中转录的时间、位置和水平的精确性和准确性的机制的看法的。
During development, genes are transcribed at specific times, locations and levels. In recent years, the emergence of quantitative tools has significantly advanced our ability to measure transcription with high spatiotemporal resolution in vivo. Here, we highlight recent studies that have used these tools to characterize transcription during development, and discuss the mechanisms that contribute to the precision and accuracy of the timing, location and level of transcription. We attempt to disentangle the discrepancies in how physicists and biologists use the term ‘precision' to facilitate interactions using a common language. We also highlight selected examples in which the coupling of mathematical modeling with experimental approaches has provided important mechanistic insights, and call for a more expansive use of mathematical modeling to exploit the wealth of quantitative data and advance our understanding of animal transcription. Summary: This Review highlights how high-resolution quantitative tools and theoretical models have formed our current view of the mechanisms determining precision and accuracy in the timing, location and level of transcription in the Drosophila embryo.
影响因子：
8
作者：
Chopra, Vivek S.
通讯作者：
Chopra, Vivek S.