Investigations of the Interactions between Synthetic Antimicrobial Polymers and Substrate-Supported Lipid Bilayers Using Sum Frequency Generation Vibrational Spectroscopy
Investigations of the Interactions between Synthetic Antimicrobial Polymers and Substrate-Supported Lipid Bilayers Using Sum Frequency Generation Vibrational Spectroscopy
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DOI：
10.1021/ac1025804
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发表时间：
2011-02-15
影响因子：
7.4
通讯作者：
Chen, Zhan
中科院分区：
文献类型：
--
作者：
Avery, Christopher W.;Palermo, Edmund F.;Chen, Zhan
和频发生（SFG）振动光谱被用来分析固体支持的脂质双层作为模型的细胞膜和几个膜活性的无规共聚物与不同的亲脂性侧链，命名为OR（无基团），33 Me（甲基），11 Bz（苄基），和33 Bu（丁基）之间的相互作用，根据身份和百分比的聚合物内的侧链。进行最低抑菌浓度（MIC）和溶血浓度的生物学试验。SFG的固有表面灵敏度允许独立监测同位素标记的脂质双层小叶作为浓度的函数，以研究聚合物双层相互作用机制。对于每种共聚物，定量测定每个双层小叶被破坏的浓度。光谱证据的相互作用与双层低于临界浓度观察到的11 Bz聚合物。发现33Bu聚合物的亲脂性丁基侧链平行于表面法线取向。这项研究表明，SFG是一个有用的分析技术，提供了独特的细节，这些膜活性共聚物和脂质双层的相互作用机制。
Sum frequency generation (SFG) vibrational spectroscopy was used to analyze interactions between solid-supported lipid bilayers acting as models for cellular membranes and several membrane-active random copolymers with different lipophilic side chains, named OR (no group), 33Me (methyl group), 11Bz (benzyl group), and 33Bu (butyl group), according to both the identity and percentage of the side chains within the polymer. Biological tests of the minimum inhibitory concentration (MIC) and hemolytic concentration were performed. The inherent surface sensitivity of SFG allowed for independent monitoring of isotopically labeled lipid bilayer leaflets as a function of concentration to study polymer bilayer interaction mechanisms. Concentrations at which each bilayer leaflet was disrupted were quantitatively determined for each copolymer. Spectroscopic evidence of interaction with the bilayer below the critical concentrations was observed for the 11Bz polymer. The lipophilic butyl side chain of the 33Bu polymer was found to be oriented parallel to the surface normal. This research shows that SFG is a useful analytical technique which provides unique details regarding the interaction mechanisms of these membrane-active copolymers and lipid bilayers.