A covalently crosslinked silk fibroin hydrogel using enzymatic oxidation and chemoenzymatically synthesized copolypeptide crosslinkers consisting of a GPG tripeptide motif and tyrosine: control of gelation and resilience
A covalently crosslinked silk fibroin hydrogel using enzymatic oxidation and chemoenzymatically synthesized copolypeptide crosslinkers consisting of a GPG tripeptide motif and tyrosine: control of gelation and resilience
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DOI：
10.1039/d0py00187b
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发表时间：
2020-05
影响因子：
4.6
通讯作者：
H. Sogawa;T. Katashima;K. Numata
中科院分区：
文献类型：
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作者：
H. Sogawa;T. Katashima;K. Numata
以甘氨酸-脯氨酸-甘氨酸(GPG)三肽基序和酪氨酸(Y)为连接物，通过化学酶法合成了共价交联型丝素蛋白水凝胶。加入聚(GPGn-r-ym)可以有效地将ChemSF的凝胶时间缩短到100秒以下，而不会损失韧性。此外，由于聚(GPGn-r-ym)交联剂的灵活构象，在66%的应变下，ChemSF的弹性可提高到86%，与弹性蛋白相当。通过使用多功能多肽交联剂，可以同时达到加速胶凝和提高弹性的目的。
A covalently crosslinked silk fibroin hydrogel (ChemSF) was successfully formed via an enzymatic crosslinking reaction using chemoenzymatically synthesized copolypeptides [poly(GPGn-r-Ym)], which consist of a glycine–proline–glycine (GPG) tripeptide motif and tyrosine (Y), as linker molecules. The gelation time of ChemSF was effectively shortened to less than 100 seconds without loss of toughness by adding poly(GPGn-r-Ym). In addition, the resilience of ChemSF was improved up to 86% at 66% strain, comparable to that of elastin, due to the flexible conformation of the poly(GPGn-r-Ym) crosslinker. The acceleration of the gelation and the improvement of the resilience of ChemSF were simultaneously achieved by using a multifunctional peptide crosslinker.