Effects of microneedle length, density, insertion time and multiple applications on human skin barrier function: Assessments by transepidermal water loss
Effects of microneedle length, density, insertion time and multiple applications on human skin barrier function: Assessments by transepidermal water loss
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DOI：
10.1016/j.tiv.2010.08.012
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发表时间：
2010-10-01
影响因子：
3.2
通讯作者：
Meidan, Victor M.
中科院分区：
文献类型：
--
作者：
Gomaa, Yasmine A.;Morrow, Desmond I. J.;Meidan, Victor M.
近年来，微针（MN）阵列由于其促进有效透皮药物递送的能力而引起了相当大的关注。尽管有可观的研究，仍然有争议的不同MN尺寸或应用模式如何影响透化。本研究旨在通过在插入固体聚合物MN后对皮肤切片的人类皮肤样品进行经表皮水分损失测量来研究这个问题。插入引起屏障功能的初始急剧下降，随后是较慢的不完全恢复-与随后由于皮肤弹性而收缩的MN-代微通道一致的范例。虽然600 μ m长的MN比400 μ m长的MN对皮肤的干扰更大，但插入1000 μ m长的MN引起完整性的较小初始下降，随后是一定程度的长期透化。这可以解释为最长的针压缩组织，然后在随后的几个小时内减压。多次插入具有与增加MN长度类似的效果。有一些证据表明，增加MN密度抑制由组织收缩引起的部分屏障恢复。将MN嵌入皮肤似乎减少了屏障功能的初始插入后下降。我们的研究结果表明，这种体外TEWL方法可用于快速筛选MN对皮肤的影响。(C)2010爱思唯尔有限公司版权所有。
Microneedle (MN) arrays have attracted considerable attention in recent years due to their ability to facilitate effective transdermal drug delivery. Despite appreciable research, there is still debate about how different MN dimensions or application modes influence permeabilization. This study aimed to investigate this issue by taking transepidermal water-loss measurements of dermatomed human skin samples following the insertion of solid polymeric MNs. Insertions caused an initial sharp drop in barrier function followed by a slower incomplete recovery - a paradigm consistent with MN-generation of microchannels that subsequently contract due to skin elasticity. While 600 pm-long MNs were more skin-perturbing than 400 pm MNs, insertion of 1000 pm-long MNs caused a smaller initial drop in integrity followed by a degree of long term permeabilization. This is explainable by the longest needles compacting the tissue, which then decompresses over subsequent hours. Multiple insertions had a similar effect as increasing MN length. There was some evidence that increasing MN density suppressed the partial barrier recovery caused by tissue contraction. Leaving MNs embedded in skin seemed to reduce the initial post-insertion drop in barrier function. Our results suggest that this in vitro TEWL approach can be used to rapidly screen MN-effects on skin. (C) 2010 Elsevier Ltd. All rights reserved.