Consideration of dipole orientation angles yields accurate rate equations for energy transfer in the rapid diffusion limit.
Consideration of dipole orientation angles yields accurate rate equations for energy transfer in the rapid diffusion limit.
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考虑偶极子取向角可得出快速扩散极限内能量传递的准确速率方程。
DOI：
10.1016/s0006-3495(92)81968-9
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发表时间：
1992
影响因子：
3.4
通讯作者：
Steel,DG
中科院分区：
文献类型：
--
作者：
Mersol,JV;Wang,H;Gafni,A;Steel,DG
合适的供体和受体发色团之间的偶极-偶极能量转移是一种重要的发光猝灭机制，并已被证明可用于分子水平上的距离测定。在快速扩散极限中，供体的激发态寿命足够长，允许供体和受体在去激发之前扩散许多倍于它们的平均分离，通常假设相对偶极取向由于旋转布朗运动而完全平均。在这种简化的假设下，分析表达式已经推导出较早的供体和受体之间的能量转移速率，其特征在于不同的几何形状。然而，大多数这样的表达式仅仅是近似的，因为完全的角度平均仅在围绕每个发色团具有球对称性的几何结构中才被允许。本文分析表达式，正确地占不完整的角度平均由于空间位阻的几种几何形状。每个方程揭示了能量转移速率对发色团取向的依赖性。结果表明，正确地考虑这种影响，可以导致改进的估计距离最近的方法从测得的淬火率的基础上能量转移实验。
Dipole-dipole energy transfer between suitable donor and acceptor chromophores is an important luminescence quenching mechanism and has been shown to be useful for distance determination at the molecular level. In the rapid diffusion limit, where the excited-state lifetime of the donor is long enough to allow the donor and acceptor to diffuse many times their average separation before deexcitation, it is usually assumed that the relative dipolar orientation is completely averaged due to rotational Brownian motion. Under this simplifying assumption, analytical expressions have been derived earlier for the energy transfer rate between donor and acceptor characterized by different geometries. Most such expressions, however, are only approximate because complete angular averaging is permitted only in a geometry that possesses spherical symmetry surrounding each chromophore. In this paper analytical expressions that correctly account for incomplete angle averaging due to steric hindrance are presented for several geometries. Each of the equations reveals a dependence of the energy transfer rate on chromophore orientation. It is shown that correctly accounting for this effect can lead to improvements in estimates of the distance of closest approach from measured quenching rates based on energy transfer experiments.
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DOI：
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发表时间：
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期刊：
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DOI：
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期刊：
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影响因子：
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影响因子：
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作者：
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作者：
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