Relationship between the glutathione-responsive degradability of thiol-organosilica nanoparticles and the chemical structures
Relationship between the glutathione-responsive degradability of thiol-organosilica nanoparticles and the chemical structures
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DOI：
10.1557/jmr.2018.501
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发表时间：
2019-04-15
影响因子：
2.7
通讯作者：
Nakamura, Michihiro
中科院分区：
文献类型：
--
作者：
Doura, Tomoshiro;Nishio, Tadashi;Nakamura, Michihiro
刺激响应可降解二氧化硅纳米颗粒（NPs）是纳米材料研究的活跃课题，因为它们有望成为能够控制药物释放的低健康风险纳米载体。在各种刺激响应的二氧化硅NPs中，含二硫键的NPs可被谷胱甘肽还原形式（GSH）降解。本文合成并表征了3-巯基丙基三甲氧基硅烷（MPMS）和3-巯基丙基（二甲氧基）甲基硅烷（MPDMS）制备的三种巯基有机硅NPs。MPMS NPs、MPDMS NPs和MPMS-MPDMS杂交NPs的结果表明，随着MPDMS在硫醇-有机硅NPs中含量的增加，硫醇二硫键的丰度比增加。我们还通过电子显微镜和Ellman的测试揭示了具有二硫键的硫醇-有机二氧化硅NPs是gsh响应的可降解二氧化硅NPs。此外，我们合成了荧光MPMS-MPDMS NPs，包括罗丹明B，并证明了NPs中染料的gsh响应释放。这些实验表明，巯基有机二氧化硅NPs具有二硫键作为gsh反应性药物载体的潜力。
Stimuli-responsive degradable silica nanoparticles (NPs) are active topics of nanomaterial research, because they are expected to be low health-risk nanocarriers capable of controlled release of drugs. Among various stimuli-responsive silica NPs, disulfide bond-containing NPs show degradability by glutathione reduced form (GSH). Here, we synthesized and characterized three kinds of thiol-organosilica NPs made from 3-mercaptopropyltrimethoxysilane (MPMS) and 3-mercaptopropyl(dimethoxy) methylsilane (MPDMS). MPMS NPs, MPDMS NPs, and MPMS-MPDMS hybrid NPs revealed that the abundance ratio of disulfide bonds to thiols increased with the increase in content rate of MPDMS in thiol-organosilica NPs. We also revealed that thiol-organosilica NPs, which have disulfide bonds, are GSH-responsive degradable silica NPs using an electron microscopy and Ellman's tests. Furthermore, we synthesized fluorescent MPMS-MPDMS NPs, including rhodamine B, and demonstrated the GSH-responsive release of dye from the NPs. These experiments indicate the potential of thiol-organosilica NPs, which have disulfide bonds as a GSH-responsive drug carrier.