Chemo-immunological studies on conjugated carbohydrate-proteins II. Immunological specificity of synthetic sugar-protein antigens
Chemo-immunological studies on conjugated carbohydrate-proteins II. Immunological specificity of synthetic sugar-protein antigens
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DOI：
10.1084/jem.50.4.533
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发表时间：
1929-10-01
影响因子：
15.3
通讯作者：
Goebel, WF
中科院分区：
文献类型：
--
作者：
Avery, OT;Goebel, WF
1. 当两种化学性质不同的碳水化合物衍生物与同一蛋白质结合时，新形成的抗原表现出不同的免疫特异性。2. 当相同的碳水化合物自由基与两种化学性质不同且血清学上不同的蛋白质结合时，这两种糖蛋白由此形成的糖蛋白获得了共同的血清学特异性。3. 人工制备的糖蛋白新获得的特异性是由附着在蛋白质分子上的碳水化合物自由基的化学构成决定的。当葡萄糖和半乳糖这两种异构体的相应糖苷偶联到相同的蛋白质上时，其分子结构的简单差异就足以确定蛋白质的特异性。4. 未偶联的糖苷虽然在免疫血清中不沉淀，但能抑制同源糖蛋白与其特异性抗体之间的反应。抑制试验是特定的。5. 不与蛋白质结合的糖衍生物具有碳水化合物半抗原的性质；它们不具有抗原性，但如抑制试验所示，与含有同源重氮化糖苷的蛋白质诱导的抗体具有特异性反应性。6. 结合含复合糖的细菌抗原的化学免疫学性质，讨论了人工制备糖蛋白的特异性。
1. When two chemically different carbohydrate derivatives are bound to the same protein, the newly formed antigens exhibit distinct immunological specificity. 2. When the same carbohydrate radical is conjugated with two chemically different and serologically distinct proteins both of the sugar-proteins thus formed acquire a common serological specificity. 3. The newly acquired specificity of the artificially prepared sugar-proteins is determined by the chemical constitution of the carbohydrate radical attached to the protein molecules. Simple differences in the molecular configuration of the two isomers,—glucose and galactose—suffice to orientate protein specificity when the corresponding glucosides of the two sugars are coupled to the same protein. 4. The unconjugated glucosides, although themselves not precipitable in immune serum, inhibit the reaction between the homologous sugar-protein and its specific antibody. The inhibition test is specific. 5. The sugar derivatives unattached to protein exhibit the properties of carbohydrate haptens; they are non-antigenic but specifically reactive, as shown by inhibition tests, with antibodies induced by proteins containing the homologous diazotized glucoside. 6. The specificity of artificially prepared sugar-proteins is discussed with reference to the chemo-immunological nature of the bacterial antigens containing complex sugars.