Experimental reinfection of BALB/c mice with different recombinant type I/III strains of Toxoplasma gondii: involvement of IFN-³ and IL-10
Experimental reinfection of BALB/c mice with different recombinant type I/III strains of Toxoplasma gondii: involvement of IFN-³ and IL-10
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DOI：
10.1590/s0074-02762009000200017
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发表时间：
2009-03-01
期刊：
影响因子：
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通讯作者：
Vitor, Ricardo Wagner Almeida
中科院分区：
文献类型：
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作者：
Brandão, Geane Peroni;Melo, Maria Norma;Vitor, Ricardo Wagner Almeida
为了评估不同弓形虫株对BALB/c小鼠的再感染，用无毒力的D8株初次感染动物，并用强毒的重组株攻击动物。攻毒后30天，从存活的BALB/c小鼠中获得脑囊肿，并接种于Swiss小鼠以获得速殖子，用于DNA提取和PCR-RFLP分析以区分不同的T.弓形虫菌株存在于可能的合并感染中。通过检测T细胞产生的IFN-γ和IL-10以及测量血清样品中的免疫球蛋白水平，在D8致敏的BALB/c小鼠中评价抗弓形虫免疫应答。PCR-RFLP结果表明，BALB/c小鼠在初次感染后45天（dpi）再次感染EGS株，在180 dpi再次感染EGS和CH 3株。在D8感染后检测到高水平的IFN-γ，在45和180天间隔之间没有显著差异。然而，更高的IL-10水平和更高的血浆IgG 1和伊加检测从感染后180天获得的样品。BALB/c小鼠对不同重组T.弓形虫菌株和这种敏感性与IL-10的产生增强。
To assess reinfection of BALB/c mice with different Toxoplasma gondii strains, the animals were prime infected with the non-virulent D8 strain and challenged with virulent recombinant strains. Thirty days after challenge, brain cysts were obtained from surviving BALB/c mice and inoculated in Swiss mice to obtain tachyzoites for DNA extraction and PCR-RFLP analysis to distinguish the different T. gondii strains present in possible co-infections. Anti-Toxoplasma immune responses were evaluated in D8-primed BALB/c mice by detecting IFN-gamma and IL-10 produced by T cells and measuring immunoglobulin levels in serum samples. PCR-RFLP demonstrated that BALB/c mice were reinfected with the EGS strain at 45 days post prime infection (dpi) and with the EGS and CH3 strains at 180 dpi. High levels of IFN-gamma were detected after D8 infection, with no significant difference between 45 and 180-day intervals. However, higher IL-10 levels and higher plasmatic IgG1 and IgA were detected from samples obtained 180 days after infection. BALB/c mice were susceptible to reinfection with different recombinant T. gondii strains and this susceptibility correlated with enhancement of IL-10 production.