Circulating autoantibody to ABCC3 may be a potential biomarker for esophageal squamous cell carcinoma
Circulating autoantibody to ABCC3 may be a potential biomarker for esophageal squamous cell carcinoma
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DOI：
10.1007/s12094-012-0941-9
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发表时间：
2013-05-01
影响因子：
3.4
通讯作者：
Liu, L.
中科院分区：
文献类型：
--
作者：
Cheng, Y.;Xu, J.;Liu, L.
目前迫切需要鉴定用于食管癌早期诊断和预后的生物标志物。为探讨循环中抗ATP结合盒C3（ABCC 3）转运体自身抗体是否可作为恶性肿瘤的生物标志物，采用酶联免疫吸附试验（ELISA）检测了114例食管鳞状细胞癌（ESCC）患者和226例年龄和吸烟史相匹配的健康受试者的循环中ABCC 3自身抗体。Whitney U检验显示食管鳞癌组伊加抗体水平显著高于对照组（Z =-4.226，P < 0.001），而IgG抗体水平两组间差异无显著性（Z =-1.072，P = 0.284）。伊加检测特异性> 95%的敏感性为13.2%，检测间偏差为13.0%，IgG检测特异性> 95%的敏感性为7.9%，检测间偏差为9.4%，提示循环中抗ABCC 3的伊加自身抗体可能是食管鳞癌的一个潜在的生物标志物，可用于恶性肿瘤的早期诊断和预后判断。
There is an urgent need to identify biomarkers for early diagnosis and prognosis of esophageal cancer. The present study was undertaken to test whether circulating autoantibodies to ATP-binding cassette C3 (ABCC3) transporter could serve as a biomarker for the malignant tumor.An enzyme-linked immunosorbent assay approach was developed in-house to test circulating autoantibodies to ABCC3 in 114 patients with esophageal squamous cell carcinoma (ESCC) and 226 healthy subjects well matched in age and smoking history.Mann-Whitney U test showed that the IgA antibody levels were significantly higher in patients with ESCC than control subjects (Z = -4.226, P < 0.001) while the IgG antibody levels were not significantly different between the two groups (Z = -1.072, P = 0.284). The sensitivity against > 95 % specificity was 13.2 % for the IgA assay with an inter-assay deviation of 13.0 and 7.9 % for the IgG assay with an inter-assay deviation of 9.4 %.This work suggests that circulating IgA autoantibody to ABCC3 may be a potential biomarker for ESCC, which could be used for early diagnosis and prognosis of the malignant tumor.