Crystal structure of B-subtilis YjcG characterizing the YjcG-like group of 2H phosphoesterase superfamily
Crystal structure of B-subtilis YjcG characterizing the YjcG-like group of 2H phosphoesterase superfamily
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DOI：
10.1002/prot.22093
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发表时间：
2008-08-15
影响因子：
2.9
通讯作者：
Su, Xiao-Dong
中科院分区：
文献类型：
--
作者：
Li, Dan;Liu, Cong;Su, Xiao-Dong
2 H磷酸酯酶超家族首先由Mazumder等人1鉴定。该酶超家族共享一个共同的催化位点，并保留了环状磷酸二酯酶的活性，尽管它们的蛋白质序列极其多样化。该超家族以活性位点中来自两个保守的四肽基序HX（T/S）X（X，疏水残基）的两个His残基命名。定点突变表明，这两个His残基是酶活性所必需的。2保守的两个HX（T/S）X基序是该超家族的典型特征。酶的超家族普遍存在于几乎所有已知的物种中。它们甚至可以追溯到参与RNA代谢的所有已知生命形式的最后一个普遍共同祖先（LUCA），并随后进化为几种不同的生物学角色。1 2 H磷酸酯酶超家族可分为四大类和几个不同的成员。四个主要的组包括古细菌LigT样组、真核病毒LigT样组、YjcG样组和mlr 3352样组。1在北京大学进行的结构基因组学研究中，我们选择枯草芽孢杆菌YjcG基因作为靶基因之一，因为它与任何结构已知蛋白的同源性都很低。3这里我们给出了B的晶体结构。subtilis YjcG是YjcG样群的原型。作为第一个三维结构的群体和第一个2 H磷酸酯酶从细菌，它有助于完成结构景观
The 2H phosphoesterase superfamily was first identified by Mazumder et al. 1 This superfamily of enzymes shares a common catalytic site and preserves the activity of cyclic phosphodiesterase, although their protein sequences are extremely diversified. The superfamily was named after the two His residues from two conserved tetrapeptide motifs, HX (T/S) X (X, a hydrophobic residue), in the active site. Site-directed mutagenesis indicated that the two His residues were essential for the enzyme activity. 2 The conserved two HX (T/S) X motifs is the typical characteristic of the superfamily. The superfamily of enzymes ubiquitously exists in almost all known species. They are even traceable to the last universal common ancestor (LUCA) of all known life forms involving in RNA metabolism, and have subsequently evolved to take several distinct biological roles. 1The 2H phosphoesterase superfamily has been classified into four major groups and a few divergent members. The four major groups include the archaeo-bacterial LigT-like group, the eukaryotic-viral LigT-like group, the YjcG-like group, and the mlr3352-like group. 1 We have selected the Bacillus subtilis YjcG gene as one of the target genes due to its low homology to any structural known proteins during a structural genomics investigation at Peking University. 3 Here we present the crystal structure of B. subtilis YjcG which is the archetype of the YjcG-like group. As the first three-dimensional structure of the group and the first 2H phosphoesterase from bacteria, it contributes to complete the structural landscape