Extracellular polymers in granular sludge from different upflow anaerobic sludge blanket (UASB) reactors
Extracellular polymers in granular sludge from different upflow anaerobic sludge blanket (UASB) reactors
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DOI：
10.1007/bf00902757
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发表时间：
1994-11
影响因子：
5
通讯作者：
J. Schmidt;B. Ahring
中科院分区：
文献类型：
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作者：
J. Schmidt;B. Ahring
采用热萃取法对厌氧升流式反应器颗粒污泥中胞外聚合物（ECP）进行了定量分析。提取的最佳时间被确定为细胞内材料由于细胞裂解而对提取的细胞外材料产生显著贡献之前所需的时间。颗粒污泥的ECP含量在41 ~ 92 mg · g-1之间，主要取决于颗粒污泥的种类。对提取的ECP中的多糖、蛋白质和脂类含量进行了定量分析。此外，测定并定量了脂质的不同甲酯。较低量的多糖和蛋白质被发现在细胞外物质从颗粒上生长的产甲烷和产乙酸基质相比，颗粒上生长的更复杂的基板。相反，脂质含量较低的复杂基板。将上流式厌氧污泥床反应器的进料从含糖废水改变为含有乙酸盐、丙酸盐和丁酸盐的合成废水导致蛋白质和多糖含量的降低以及胞外物质的脂质含量的增加。此外，在嗜温条件下发现的ECP中的蛋白质和多糖的量显着高于在嗜热条件下，而脂质含量较低。
Thermal extraction was used to quantify extracellular polymers (ECP) in granules from anaerobic upflow reactors. The optimal time for extraction was determined as the time needed before the intracellular material gives a significant contribution to the extracted extracellular material due to cell lysis. ECP contents of 41 to 92 mg · g−1volatile suspended solids of granules were found depending on the type of granular sludge examined. The content of polysaccharides, protein and lipids in the extracted ECP was quantified. Furthermore, the different methyl esters of the lipids were determined and quantified. Lower amounts of polysaccharides and proteins were found in the extracellular material from granules grown on methanogenic and acetogenic substrates compared to granules grown on more complex substrates. In contrast, the lipid content was lower on complex substrates. Changing the feed of an upflow anaerobic sludge blanket reactor from a sugar-containing waste-water to a synthetic waste-water containing acetate, propionate and butyrate resulted in a decrease in both the protein and polysaccharide content and an increase in the lipid content of the extracellular material. Furthermore, the amount of protein and polysaccharides in the ECP found under mesophilic conditions was significantly higher than under thermophilic conditions, while the lipid content was lower.