Life-span and size of the trans-membrane channel formed by large doses of complement.
Life-span and size of the trans-membrane channel formed by large doses of complement.
复制标题
大剂量补体形成的跨膜通道的寿命和大小。
DOI：
10.4049/jimmunol.124.5.2281
复制
发表时间：
1980
影响因子：
4.4
通讯作者：
M. Mayer
中科院分区：
文献类型：
--
作者：
L. Ramm;M. Mayer
为了评价补体（C）处理膜中跨膜通道的寿命和大小，用抗Forssman抗体和大剂量豚鼠C处理含有捕获的天然蛋白标记物以及残留血红蛋白的重新密封的红细胞血影。卵清蛋白和血红蛋白通过这样产生的通道缓慢释放，而人血清白蛋白则不然。血红蛋白的释放不被细胞外牛血清白蛋白阻断。血红蛋白的释放在4 ℃下持续至少72小时。卵清蛋白或血红蛋白释放的半对数图显示速率常数逐渐减小，这表明在实验期间通道的缓慢损失。这些实验表明，红细胞血影膜中产生的通道大剂量的C有一个很长的，虽然有限的寿命。基于卵清蛋白和血红蛋白的释放，它们的有效直径至少为55 A，并且不超过150 A，因为血清白蛋白不被释放。然而，根据其他人的电子显微镜观察，100 A的上限更合理。这些结果与甜甜圈模型是一致的。
To evaluate the life-span and size of trans-membrane channels in complement (C) treated membranes, resealed erythrocyte ghosts containing trapped native protein markers, as well as residual hemoglobin, were treated with anti-Forssman antibody and large doses of guinea pig C. Ovalbumin and hemoglobin were released slowly through the channels so produced, whereas human serum albumin was not. Release of hemoglobin was not blocked by extracellular bovine serum albumin. Release of hemoglobin continued for at least 72 hr at 4 degrees C. Semi-logarithmic plots of ovalbumin or hemoglobin release showed gradual diminution of the rate constant, which indicates slow loss of channels during the experimental period. These experiments demonstrate that the channels produced in erythrocyte ghost membranes by large C doses have a long, although finite, life-span. Their effective diameter is al least 55 A on the basis of ovalbumin and hemoglobin release, and not more than 150 A, since serum albumin was not released. However, an upper limit of 100 A would be more reasonable in light of electronmicroscopic observations by others. These results are compatible with the doughnut model.