Comparative and functional genomic analyses of iron transport and regulation in Leptospira spp.
Comparative and functional genomic analyses of iron transport and regulation in Leptospira spp.
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DOI：
10.1128/jb.00711-06
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发表时间：
2006-11-01
影响因子：
3.2
通讯作者：
Picardeau, M.
中科院分区：
文献类型：
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作者：
Louvel, H.;Bommezzadri, S.;Picardeau, M.
钩端螺旋体属的螺旋体含有腐生和致病成员，后者是钩端螺旋体病的原因。尽管最近对病原体问号钩端螺旋体的基因组进行了测序，但这些细菌的生长缓慢、它们对人类的毒力以及遗传工具的缺乏使得研究这些病原体变得困难。相比之下，针对腐生菌 L. biflexa 的多种遗传工具的开发使其能够用作模式细菌。钩端螺旋体属生长需要铁。在这项工作中，我们展示了钩端螺旋体属。可以从不同来源获取铁，包括铁载体。本研究对腐生菌双折乳杆菌和病原体问号乳杆菌的铁吸收系统及其调节进行了比较基因组分析。例如，我们的数据表明，L. biflexa 和 L. interrogans 分别含有 8 个和 12 个基因，其产物与已证明是 TonB 依赖性受体的蛋白质具有同源性。我们发现一些参与铁吸收的基因在铁反应中存在差异表达。此外，我们还能够破坏双弯乳杆菌中与铁获取系统或铁调节有关的几个假定基因。比较基因组学与基因失活相结合，为我们提供了有关钩端螺旋体属铁稳态的重要功能信息。
The spirochetes of the Leptospira genus contain saprophytic and pathogenic members, the latter being responsible for leptospirosis. Despite the recent sequencing of the genome of the pathogen L. interrogans, the slow growth of these bacteria, their virulence in humans, and a lack of genetic tools make it difficult to work with these pathogens. In contrast, the development of numerous genetic tools for the saprophyte L. biflexa enables its use as a model bacterium. Leptospira spp. require iron for growth. In this work, we show that Leptospira spp. can acquire iron from different sources, including siderophores. A comparative genome analysis of iron uptake systems and their regulation in the saprophyte L. biflexa and the pathogen L. interrogans is presented in this study. Our data indicated that, for instance, L. biflexa and L. interrogans contain 8 and 12 genes, respectively, whose products share homology with proteins that have been shown to be TonB-dependent receptors. We show that some genes involved in iron uptake were differentially expressed in response to iron. In addition, we were able to disrupt several putative genes involved in iron acquisition systems or iron regulation in L. biflexa. Comparative genomics, in combination with gene inactivation, gives us significant functional information on iron homeostasis in Leptospira spp.