BEDTools: The Swiss-Army Tool for Genome Feature Analysis.
BEDTools: The Swiss-Army Tool for Genome Feature Analysis.
复制标题
DOI：
10.1002/0471250953.bi1112s47
复制
发表时间：
2014-09-08
影响因子：
--
通讯作者：
Quinlan, Aaron R
中科院分区：
文献类型：
--
作者：
Quinlan, Aaron R
技术的进步使得DNA测序作为一种灵活的工具来表征遗传变异和测量不同的细胞现象，如基因亚型表达和转录因子结合的活性成为可能。从这些进步所实现的实验中提取生物学见解需要分析大型多维数据集。本单元介绍了使用BEDTools工具包探索高通量基因组学数据集。我提出了几个常见的基因组分析协议，并演示了如何简单的BEDTools操作可以结合起来，以创建定制的管道解决复杂的问题。
Technological advances have enabled the use of DNA sequencing as a flexible tool to characterize genetic variation and to measure the activity of diverse cellular phenomena such as gene isoform expression and transcription factor binding. Extracting biological insight from the experiments enabled by these advances demands the analysis of large, multi-dimensional datasets. This unit describes the use of the BEDTools toolkit for the exploration of high-throughput genomics datasets. I present several protocols for common genomic analyses and demonstrate how simple BEDTools operations may be combined to create bespoke pipelines addressing complex questions.