An artificial CuII complex with intriguing oxygen radical-quenching profile. X-ray structure, cytochrome c assay, and ESR study.
An artificial CuII complex with intriguing oxygen radical-quenching profile. X-ray structure, cytochrome c assay, and ESR study.
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一种具有有趣的氧自由基猝灭特性的人造 CuII 络合物。
DOI：
10.1016/0968-0896(96)00161-7
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发表时间：
1996
影响因子：
3.5
通讯作者：
Y. Sugiura
中科院分区：
文献类型：
--
作者：
M. Otsuka;H. Satake;S. Murakami;M. Doi;T. Ishida;M. Shibasaki;Y. Sugiura
合成了一种由一个吡啶和两个组氨酸单元组成的新型人工多肽HPH-PEP。X-射线单晶分析表明，HPh-PEP-CuII络合物具有独特的五配位结构，ESR谱表明其具有清除超氧阴离子的活性。用细胞色素c法和ESR自旋捕捉法对HPh-Pep-CuII的超氧猝灭谱进行了详细的研究，发现：(1)HPh-Pep-CuII不清除过氧化氢或羟基自由基，因此其清除活性是超氧阴离子所特有的；(2)HPh-Pep-CuII清除超氧阴离子时不产生过氧化氢。
A novel artificial peptide named HPH-Pep, comprising a pyridine and two histidine units, was synthesized. The HPH-Pep-CuIIcomplex had unique pentacoordinated structure as shown by X-ray crystallography and exhibited superoxide-scavenging activity as indicated by ESR spectroscopy. The superoxide-quenching profile of HPH-Pep-CuIIwas studied in detail by cytochrome c assay and ESR spin trapping and it was found that (1) HPH-Pep-CuIIdid not scavenge hydrogen peroxide or hydroxyl radical and hence the scavenging activity was specific to superoxide, and (2) HPH-Pep-CuIIdid not generate hydrogen peroxide upon scavenging superoxide.