Distinguishing species
Distinguishing species
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DOI：
10.1261/rna.617107
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发表时间：
2007-09-01
期刊：
影响因子：
4.5
通讯作者：
Wolf, Matthias
中科院分区：
文献类型：
--
作者：
Mueller, Tobias;Philippi, Nicole;Wolf, Matthias
如果有两种生物，我们如何区分它们是否属于同一物种？这对于两个不同的生物体来说可能很简单，但对于密切相关的生物体来说可能非常困难和费力。因此，一种能给出明确区分的分子标记将具有巨大的益处。内转录间隔区2（ITS2）已被广泛用于低水平的系统发育分析。案例研究表明，补偿性碱基变化（CBC）的螺旋II或螺旋III ITS2二级结构之间的两种生物与性不相容。我们分析了1300多个密切相关的物种，以测试这种相关性是否普遍适用。在93%的情况下，在同一属内分类的生物体之间发现CBC，它们属于不同的种。因此，ITS2序列结构比对中的CBC是区分甚至密切相关物种的充分条件。
Given two organisms, how can one distinguish whether they belong to the same species or not? This might be straightforward for two divergent organisms, but can be extremely difficult and laborious for closely related ones. A molecular marker giving a clear distinction would therefore be of immense benefit. The internal transcribed spacer 2 (ITS2) has been widely used for low-level phylogenetic analyses. Case studies revealed that a compensatory base change (CBC) in the helix II or helix III ITS2 secondary structure between two organisms correlated with sexual incompatibility. We analyzed more than 1300 closely related species to test whether this correlation is generally applicable. In 93%, where a CBC was found between organisms classified within the same genus, they belong to different species. Thus, a CBC in an ITS2 sequence-structure alignment is a sufficient condition to distinguish even closely related species.