Kandeliimicrobium roseum gen. nov., sp nov., a new member of the family Rhodobacteraceae isolated from mangrove rhizosphere soil
Kandeliimicrobium roseum gen. nov., sp nov., a new member of the family Rhodobacteraceae isolated from mangrove rhizosphere soil
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Kandeliimicrobium Roseum gen.
DOI：
10.1099/ijsem.0.002773
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发表时间：
2018
影响因子：
2.8
通讯作者：
Xu Ying
中科院分区：
文献类型：
--
作者：
Wang Kai Ling;Song Zhi Man;Rong Cu Huang;Hao Ling Yun;Lai Qi Liang;Li Shuang Fei;Xu Ying
从红树植物秋茄Kandelia Candel(L.)根际土壤中分离到一株革兰氏阴性、无鞭毛、短杆状细菌，命名为XY-R6T。德鲁斯，香港米埔自然保护区。菌株XY-R6T在pH5.0-9.5(最适6.5-8.0)、8-42 °C(最适28-34 °C)、0-9.5 %(w/v)氯化钠(最适1-4 %)的条件下生长。主要的异戊二烯对苯二酚是泛醌-10。主要脂肪酸为特征8(C18 ： 1ω6c和/或C18 ： 1ω7c)(55.61 %)，C19 ： 0ω8c(21.59 %)和C16 ： 0(11.24 %)。主要的极性脂为磷脂酰甘油、磷脂酰乙醇胺、氨基脂、磷脂酰胆碱和二磷脂酰甘油。菌株XY-R6T的基因组DNAG+C 含量为69.3 摩尔%。基于16S rRNA基因序列的系统发育分析表明，XY-R6T菌株属于甲型变形杆菌属中的红杆菌家族，与文新菌属成员的基因序列相似性最高(94.594.3 %)，其次是深细菌属(94.3 %)、流感嗜血杆菌属(93.8-92.5 %)、海洋杆菌属(93.8 %)和酿酒杆菌属(93.7 %)。与科内其他属的相似性低于94.0 %。综合表型、系统发育和化学分类研究表明，菌株XY-R6Trea为红杆菌科一新属新种，命名为Kandeliimmicrobium roseumgen。Nov.，sp.11月以XY-R6T(=MCCC 1K01498T=KCTC 52266T=DSM 104294T)为模式菌株。
A Gram-stain-negative, non-flagellated, short rod-shaped bacterium, designated XY-R6T, was isolated from the rhizosphere soil of a mangrove plant,Kandelia candel(L.) Druce, in Mai Po Nature Reserve, Hong Kong. Growth of strain XY-R6Twas observed at pH 5.0–9.5 (optimum 6.5–8.0), between 8 and 42 °C (optimum 28–34 °C), and in the presence of 0–9.5 % (w/v) NaCl (optimum 1–4 %). The predominant isoprenoid quinone was ubiquinone-10. The major fatty acids were summed feature 8 (C18 : 1ω6cand/or C18 : 1ω7c) (55.61 %), C19 : 0cycloω8c(21.59 %) and C16 : 0(11.24 %). The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, aminolipid, phosphatidylcholine and diphosphatidylglycerol. The genomic DNA G+C content of strain XY-R6Twas 69.3 mol%. Phylogenetic analyses, based on 16S rRNA gene sequences, revealed that strain XY-R6Tbelonged to the familyRhodobacteraceaeof the classAlphaproteobacteriaand formed a distinct lineage, showing the highest gene sequence similarities to the members of genusWenxinia(94.5–94.3 %), followed by the generaProfundibacterium(94.3 %),Defluviimonas(93.8–92.5 %),Oceanicola(93.8 %) andCereibacter(93.7 %). Similarities to other genera within the familyRhodobacteraceaewere below 94.0 %. Based on comprehensive phenotypic, phylogenetic and chemotaxonomic characterization, it is indicated that strain XY-R6Trepresents a novel species of a new genus in the familyRhodobacteraceae, for which the nameKandeliimicrobium roseumgen. nov., sp. nov. is proposed, with XY-R6T(=MCCC 1K01498T=KCTC 52266T=DSM 104294T) as the type strain.