A critical role for Syk in endothelial cell proliferation and migration
A critical role for Syk in endothelial cell proliferation and migration
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DOI：
10.1006/bbrc.2001.5355
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发表时间：
2001-08-10
影响因子：
3.1
通讯作者：
Yamamura, H
中科院分区：
文献类型：
--
作者：
Inatome, R;Yanagi, S;Yamamura, H
Syk是一种蛋白酪氨酸激酶，在造血细胞中广泛表达，参与激活的免疫受体与下游信号的偶联。另一方面，syk缺陷小鼠在子宫内表现出严重的斑点，并在出生后不久死亡。最近我们在胚胎Syk缺陷小鼠的内皮细胞中发现了Syk的表达和这些细胞的形态学缺陷。在这里，我们报道了腺病毒介导的Syk显性阴性突变体的表达严重损害了人脐静脉内皮细胞的增殖和迁移。此外，Syk激酶活性与细胞外信号调节激酶激活密切相关。我们的研究结果表明，Syk在内皮细胞功能中起着关键作用，包括形态发生、细胞生长、迁移和存活，并有助于维持体内血管的完整性。(C) 2001学术出版社。
Syk is a protein-tyrosine kinase that is widely expressed in haematopoietic cells and involved in coupling activated immunoreceptors to downstream signaling. On the other hand, Syk-deficient mice showed severe petechiae in utero and died shortly after birth. Recently we have shown the expression of Syk in endothelial cells and morphological defects of these cells in embryonic Syk-deficient mice. Here we report that both proliferation and migration of human umbilical vein endothelial cells were severely impaired by adenovirus-mediated expression of Syk dominant negative mutants. Furthermore, a close relationship between Syk kinase activity and extracellular signal-regulated kinase activation was suggested. Our results indicate that Syk plays a critical role in endothelial cell functions, including morphogenesis, cell growth, migration, and survival, and contributes to maintaining vascular integrity in vivo. (C) 2001 Academic Press.