Urinary miR-21 as a potential biomarker of hypertensive kidney injury and fibrosis.
Urinary miR-21 as a potential biomarker of hypertensive kidney injury and fibrosis.
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尿液 miR-21 作为高血压肾损伤和纤维化的潜在生物标志物。
DOI：
10.1038/s41598-017-18175-3
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发表时间：
2017-12-18
影响因子：
4.6
通讯作者：
Weng H
中科院分区：
文献类型：
--
作者：
Chen C;Lu C;Qian Y;Li H;Tan Y;Cai L;Weng H
肾活检被认为是诊断肾脏疾病病因的金标准，但伴随着不可忽视的并发症。我们探讨了使用尿microRNA（miRNA）作为高血压肾损伤的非侵入性生物标志物的可能性。我们评估了与对照组相比，由醋酸脱氧皮质酮（DOCA）盐诱导的肾损伤的高血压小鼠的肾脏和尿液中的差异miRNA表达。与对照组相比，DOCA-盐处理分别从第2天起显著增加肾小管病变和从第4天起显著增加纤维化相关基因的mRNA表达。与对照组相比，第8天尿白蛋白和N-乙酰-β-D-氨基葡萄糖苷酶显著增加。阵列结果显示，585个miRNA中有20个在肾脏中高度表达，并且与对照组相比在第8天显著增加，包括miR-21、miR-146 b、miR-155和miR-132，通过实时聚合酶链反应证实，并且从第4天开始显著增加。从第4天开始，尿液中的miR-21/肌酐显著高于对照组，并且比尿白蛋白更早被检测到。总之，我们已经确定了与肾损伤的组织病理学病变和功能标志物相关的尿miR-21，以促进高血压肾损伤的潜在无创检测。
Kidney biopsy is considered the golden criterion for diagnosing the etiology of kidney disease but accompanied by non-negligible complications. We explored the possibility of using urinary microRNA (miRNA) as a non-invasive biomarker for hypertensive kidney injury. We assessed differential miRNA expressions in the kidneys and urine of hypertensive mice with kidney injury induced by deoxycorticosterone acetate (DOCA)-salt compared to the controls. DOCA-salt treatment significantly increased renal tubular lesions from day 2 and mRNA expression of fibrosis-related genes from day 4 compared to the controls, respectively. Urinary albumin and N-acetyl-beta-D-glucosaminidase was significantly increased on day 8 compared to the controls. Array results showed that 20 out of 585 miRNAs were highly expressed in the kidneys and significantly increased on day 8 compared to the controls, including miR-21, miR-146b, miR-155 and miR-132, which were confirmed by real-time polymerase chain reaction and were significantly higher from day 4. The miR-21/creatinine in the urine from day 4 was significantly higher than that of the controls and was detected earlier than urinary albumin. In conclusion, we have identified urinary miR-21 that correlates with histopathological lesions and functional markers of kidney damage to facilitate a potential noninvasive detection for hypertensive kidney injury.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
尿外泌体/微泡中的 MicroRNA-29c 作为肾纤维化的生物标志物
DOI：
10.1152/ajprenal.00148.2013
发表时间：
2013-10-01
影响因子：
4.2
作者：
Lv, Lin-Li;Cao, Yu-Han;Liu, Bi-Cheng
通讯作者：
Liu, Bi-Cheng
影响因子：
6.1
作者：
Gaede, Luise;Liebetrau, Christoph;Moellmann, Helge
通讯作者：
Moellmann, Helge
影响因子：
3.7
作者：
Cheng L;Quek CY;Sun X;Bellingham SA;Hill AF
通讯作者：
Hill AF
影响因子：
5.9
作者：
Preda, A;Van Dijk, LC;Pattynama, PMT
通讯作者：
Pattynama, PMT