Hydrolysis of timosaponin BII by the crude enzyme from Aspergillus niger AS 3.0739
Hydrolysis of timosaponin BII by the crude enzyme from Aspergillus niger AS 3.0739
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DOI：
10.1080/10286020.2010.510470
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发表时间：
2010-11
影响因子：
1.7
通讯作者：
Wen-Bin Zhou;Bing Feng;Hong-zhi Huang;Ping Liu;He-shui Yu;Yang Zhao;Yu-juan Qin;L. Kang;B. Ma
中科院分区：
文献类型：
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作者：
Wen-Bin Zhou;Bing Feng;Hong-zhi Huang;Ping Liu;He-shui Yu;Yang Zhao;Yu-juan Qin;L. Kang;B. Ma
Timosaponin BII(1)是一种具有潜在抗痴呆活性的甾体皂苷，它被黑曲霉as3.0739的粗酶区域选择性水解为其去糖基衍生物。通过1D NMR、2D NMR、FAB-MS、ir - esi - ms等光谱数据对三种生物转化产物timmosaponin BII-a(2)、timosaponin BII-b(3)、timosaponin BII-c(4)进行了纯化，并对其结构进行了鉴定。化合物2和3是新化合物。
Timosaponin BII (1), a steroidal saponin showing potential anti-dementia activity, was regioselectively hydrolyzed into its deglycosyl derivatives by the crude enzyme from Aspergillus niger AS 3.0739. Three biotransformation products, timosaponin BII-a (2), timosaponin BII-b (3), and timosaponin BII-c (4), were purified and their structures were elucidated on the basis of 1D NMR, 2D NMR, FAB-MS, and HR-ESI-MS spectral data. Compounds 2 and 3 are new compounds.