Experimental evidence for a proteinaceous presegmental wave required for morphogenesis of axolotl mesoderm.
Experimental evidence for a proteinaceous presegmental wave required for morphogenesis of axolotl mesoderm.
复制标题
蝾螈中胚层形态发生所需的蛋白质前节波的实验证据。
DOI：
10.1016/0012-1606(85)90390-2
复制
发表时间：
1985
影响因子：
2.7
通讯作者：
Steinberg,MS
中科院分区：
文献类型：
--
作者：
Gillespie,LL;Armstrong,JB;Steinberg,MS
中胚层的蝾螈胚胎在不同的发育阶段，短暂暴露于无钙的0.01%胰蛋白酶的解决方案，暂时去除表皮。发现这种处理破坏了局部区域的体节分割，并且原肾管无法通过该区域迁移。受影响的区域由3.91 ± 1.04个体节组成，与分裂同步，体节宽度为3.55 ± 0.69。在340 μ M钙存在下的胰蛋白酶化导致正常的导管迁移，但体节分割仍然受到影响。这些结果表明，在体节中胚层和导管路径的侧中胚层中存在胰蛋白酶敏感区域，该区域在体节分割之前行进并与体节分割同步。此外，导管路径的胰蛋白酶敏感性是钙依赖性的，而体节中胚层的胰蛋白酶敏感性不是。
Mesoderm of axolotl embryos at various developmental stages was briefly exposed to a calcium-free 0.01% trypsin solution by temporary removal of the epidermis. This treatment was found to disrupt somite segmentation in a localized region and the pronephric duct was unable to migrate through this region. The affected area, consisting of 3.91 ± 1.04 somites, traveled through the embryo in synchrony with, and 3.55 ± 0.69-somite widths ahead of segmentation. Trypsinization in the presence of 340 μMcalcium resulted in normal duct migration while somite segmentation was still affected. These results demonstrate the existence of a trypsin-sensitive region in the somitic mesoderm and the lateral mesoderm of the duct path that travels in advance of somite segmentation and in synchrony with it. In addition, the trypsin sensitivity of the duct path is calcium dependent whereas that of the somitic mesoderm is not.