Novel miniature SPR immunosensor equipped with all-in-one multi-microchannel sensor chip for detecting low-molecular-weight analytes
Novel miniature SPR immunosensor equipped with all-in-one multi-microchannel sensor chip for detecting low-molecular-weight analytes
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DOI：
10.1016/j.bios.2007.08.010
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发表时间：
2007-12-15
影响因子：
12.6
通讯作者：
Miura, Norio
中科院分区：
文献类型：
--
作者：
Kim, Sook Jin;Gobi, K. Vengatajalabathy;Miura, Norio
研究了一种用于检测低分子量毒素2，4-二氯苯氧基乙酸(2，4-D)的简单而通用的小型表面等离子体共振(SPR)免疫传感器，该传感器能够并行分析多个分析物或多个分析物样品。研制了一种特殊设计的多微通道SPR传感器模块，将镀金薄膜的光学棱镜、多微通道板(8个通道)和流通池集成在一起。通过对2，4-D偶联物的物理吸附制备了传感表面，并应用间接竞争免疫分析原理对2，4-D进行了定量。一步分析了多个2，4-D样品，建立了0.1 ppb(ngml(-1))2，4-D的低检测线性范围。便携式SPR免疫传感器对2，4-D的选择性检测能力已被证明，尽管存在各种结构相似的干扰物和河水样品。独立的一体式传感器模块非常有利于在多次测定之间进行货架存储，并且同一个多微通道流动模块可重复使用35天以上，间歇存储(4-8摄氏度)已得到证实。通过引入一种简单的基于亲和素-生物素相互作用的夹心免疫分析，可以进一步提高2，4-D的低密度脂蛋白，传感器信号极大地放大约10倍，低密度脂蛋白增加到0.008 ppb。本文所展示的用于多个样品同时分析的微型SPR传感器具有可重复使用和良好的存储能力，是推动生物传感器技术进步的重要考虑因素。(C)2007 Elsevier B.V.保留所有权利。
A simple and versatile miniaturized surface plasmon resonance (SPR) immunosensor enabling parallel analysis of multiple analytes or multiple samples of an analyte has been investigated for detection of a low-molecular-weight (lmw) toxin, 2,4-dichlorophenoxyacetic acid (2,4-D). A specially designed multi-microchannel SPR sensor module, integrating an optical-prism coated with an array of thin Au-films, a multi-microchannel plate (eight channels) and a flow-cell together, has been fabricated. The sensing surface was fabricated simply by physical adsorption of a protein conjugate of 2,4-D, and an indirect competitive immunoassay principle has been applied for the quantification of 2,4-D. Multiple 2,4-D samples were analyzed in a single step and a low-detection-linlit (LDL) of 0.1 ppb (ng ml(-1)) 2,4-D was established. Competence of the portable SPR immunosensor for selective detection of 2,4-D despite the presence of various structurally resemblant interferents and from river-water samples has been demonstrated. The independent all-in-one sensor module highly favors shelf-storage between multiple determinations, and reusability of a same multi-microchannel flow-module for more than 35 days with intermittent storage (4-8 degrees C) has been confirmed. The LDL of 2,4-D could be enhanced further by introducing a simple avidin-biotin interaction-based sandwich immunoassay, with which the sensor signal multiplied enormously by a factor of ca. 10 and the LDL enhanced to 0.008 ppb. The miniature SPR sensor demonstrated here for simultaneous analysis of multiple samples with reusability and good storage ability is an important consideration for the advancement of biosensor technology. (c) 2007 Elsevier B.V. All rights reserved.