mRNA expression of genes involved in fatty acid utilization in skeletal muscle and white adipose tissues of sows during lactation
mRNA expression of genes involved in fatty acid utilization in skeletal muscle and white adipose tissues of sows during lactation
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DOI：
10.1016/j.cbpa.2010.12.007
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发表时间：
2011-04-01
影响因子：
2.3
通讯作者：
Eder, Klaus
中科院分区：
文献类型：
--
作者：
Ringseis, Robert;Heller, Kristin;Eder, Klaus
啮齿类动物在哺乳期间能够降低肝脏和肌肉对脂肪酸的利用，以节省脂肪酸用于生产乳汁中的三酰甘油，这一作用是通过下调过氧化物酶体增殖物激活受体α (PPAR α)来介导的。本研究旨在探讨在哺乳期母猪中是否也有类似的脂肪酸节约作用。与非泌乳母猪相比，我们考虑了PPAR α及其靶基因在泌乳母猪肌肉和脂肪组织活检样本中参与脂肪酸利用。在肌肉中，参与脂肪酸利用的PPAR α靶基因在哺乳期间上调，表明肌肉中脂肪酸利用增加。PPAR α的激活可能是由于在泌乳母猪中观察到血浆中非酯化脂肪酸浓度的增加。与肌肉相比，白色脂肪组织中PPAR α及其参与β -氧化的靶基因在哺乳期早期下调。总的来说，目前的研究表明，与大鼠不同，母猪不能减少肌肉中脂肪酸的利用，以节省脂肪酸用于产奶量。然而，在哺乳早期，脂肪组织中的脂肪酸氧化降低，这可能有助于保存脂肪组织释放的脂肪酸，以便通过血液输送到其他组织，包括乳腺。(C) 2011爱思唯尔公司版权所有。
Rodents are able to lower fatty acid utilization in liver and muscle during lactation in order to spare fatty acids for the production of milk triacylglycerols, an effect which is mediated by a down-regulation of peroxisome proliferator-activated receptor alpha (PPAR alpha). The present study was performed to investigate whether similar fatty acid sparing effects are developing in lactating sows. We considered PPAR alpha and its target genes involved in fatty acid utilization in biopsy samples from muscle and adipose tissue of lactating compared to non-lactating sows. In muscle, PPAR alpha target genes involved in fatty acid utilization were up-regulated during lactation indicating that the fatty acid utilization in muscle was increased. Activation of PPAR alpha was probably due to increased concentrations of non-esterified fatty acids in plasma observed in the lactating sows. In contrast to muscle, PPAR alpha and its target genes involved in beta-oxidation in white adipose tissue were down-regulated in early lactation. Overall, the present study shows that sows, unlike rats, are not able to reduce the fatty acid utilization in muscle in order to spare fatty acids for milk production. However, fatty acid oxidation in adipose tissue is lowered during early lactation, an effect that might be helpful to conserve fatty acids released from adipose tissue for the delivery into other tissues, including mammary gland, via the blood. (C) 2011 Elsevier Inc. All rights reserved.