Mouse cells contain two distinct ras gene mRNA species that can be translated into a p21 onc protein
Mouse cells contain two distinct ras gene mRNA species that can be translated into a p21 onc protein
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小鼠细胞含有两种不同的 ras 基因 mRNA 种类，可以翻译成 p21 onc 蛋白
DOI：
10.1128/mcb.2.11.1339-1345.1982
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发表时间：
1982
影响因子：
5.3
通讯作者：
E. Scolnick
中科院分区：
文献类型：
--
作者：
R. Ellis;D. DeFeo;M. Furth;E. Scolnick
鼠肉瘤病毒的Kirsten（Ki）和Harvey（Ha）株编码21，000-道尔顿蛋白（p21 ras），其是这些病毒的转化基因的产物。正常细胞表达低水平的p21 ras，其由与Ki和Ha鼠肉瘤病毒转化基因同源的细胞基因（Ki-ras和Ha-ras）编码。骨髓来源的小鼠细胞系，416 B，已被证明表达异常高水平的p21 ras。在这篇文章中，我们研究了p21 ras基因在416 B和其他正常小鼠细胞中表达的分子生物学。我们确定了四个不同的多聚腺苷酸化和多聚核糖体相关的RNA，两个相关的Ki-ras和两个Ha-ras。在416 B细胞中，大小为5.2和2.0的Ki-ras RNA的水平都比在正常小鼠细胞中发现的水平升高了约25倍;在416 B细胞中，两种Ha-ras RNA的水平没有相应的变化。我们部分纯化了两个Ki-ras mRNA，并通过蔗糖密度梯度中的速度沉降将它们分离。5.2-和2.0-丝氨酸蛋白酶mRNA在体外都能翻译成p21 ras。这些结果表明，细胞onc蛋白可以从两种不同的细胞mRNA种类翻译。
The Kirsten (Ki) and Harvey (Ha) strains of murine sarcoma virus encode a 21,000-dalton protein (p21 ras) which is the product of the transforming gene of these viruses. Normal cells express low levels of p21 ras encoded by cellular genes (Ki-ras and Ha-ras) homologous to the Ki and Ha murine sarcoma virus transformation genes. A bone marrow-derived mouse cell line, 416B, has been shown to express unusually high levels of p21 ras. In this manuscript, we investigated the molecular biology of p21 ras gene expression in 416B and other normal mouse cells. We identified four distinct polyadenylated and polysome-associated RNAs, two related to Ki-ras and two to Ha-ras. The levels in 416B cells of the two Ki-ras RNAs, sized 5.2 and 2.0 kilobases, were both elevated approximately 25-fold over levels found in normal mouse cells; there was no corresponding change in 416B cells in the levels of the two Ha-ras RNAs. We partially purified the two Ki-ras mRNAs and separated them by velocity sedimentation in sucrose density gradients. Both the 5.2- and 2.0-kilobase mRNAs could be translated in vitro into p21 ras. These results show that a cellular onc protein can be translated from two distinct cellular mRNA species.