MOLECULAR ANALYSIS OF NATURAL LEAF SENESCENCE IN ARABIDOPSIS-THALIANA
MOLECULAR ANALYSIS OF NATURAL LEAF SENESCENCE IN ARABIDOPSIS-THALIANA
复制标题
DOI：
10.1111/j.1399-3054.1994.tb05343.x
复制
发表时间：
1994-10-01
影响因子：
6.4
通讯作者：
AMASINO, RM
中科院分区：
文献类型：
--
作者：
LOHMAN, KN;GAN, SS;AMASINO, RM
我们已经分析了大分子的变化，与拟南芥叶片自然衰老。叶绿素的损失是叶片衰老的特征，伴随着总RNA和蛋白质水平下降的特定模式。我们构建了两个代表拟南芥叶片不同衰老阶段mRNA的cDNA文库。分离出6个对应于在衰老过程中丰度显著增加的mRNA的cDNA克隆。这些mRNA的水平保持升高到衰老的后期阶段，此时叶片的大部分叶绿素和蛋白质已经降解。还鉴定了与表现出对比行为的mRNA对应的六个cDNA克隆;这些mRNA的水平在衰老期间降低至不可检测的水平。这些特定的mRNA的水平在衰老过程中的变化。结果表明，基因表达的主要变化发生在拟南芥叶片衰老过程中。
We have analyzed macromolecular changes that are associated with natural leaf senescence in Arabidopsis thaliana. The loss of chlorophyll that is characteristic of leaf senescence is accompanied by a specific pattern of decline of total RNA and protein levels. We have constructed two cDNA libraries representing mRNAs from Arabidopsis leaves at different senescence stages. Six cDNA clones corresponding to mRNAs that substantially increase in abundance during senescence were isolated. The levels of these mRNAs remain elevated into the late stages of senescence when most of the chlorophyll and protein of the leaf has been degraded. Six cDNA clones that correspond to mRNAs that exhibit contrasting behavior were also identified; the levels of these mRNAs decrease to undetectable levels during senescence. The changes in the levels of these specific mRNAs during the course of senescence are presented. The results indicate that major changes in gene expression occur in Arabidopsis leaves during the process of senescence.