Stimuli-responsive microwells for formation and retrieval of cell aggregates.
Stimuli-responsive microwells for formation and retrieval of cell aggregates.
复制标题
DOI：
10.1039/c004732e
复制
发表时间：
2010-09-21
期刊：
影响因子：
6.1
通讯作者：
Khademhosseini A
中科院分区：
文献类型：
--
作者：
Tekin H;Anaya M;Brigham MD;Nauman C;Langer R;Khademhosseini A
Generating cell aggregates is beneficial for various applications ranging from biotechnology to regenerative therapies. Previously, poly(ethylene glycol) (PEG) microwells have been demonstrated as a potentially useful method for generating controlled-size cell aggregates. In addition to controlling cell aggregate size and homogeneity, the ability to confine cell aggregates on glass adhesive substrates and subsequently retrieve aggregates from microwells for further experimentation and analysis could be beneficial for various applications. However, it is often difficult to retrieve cell aggregates from these microwells without the use of digestive enzymes. This study describes the stable formation of cell aggregates in responsive microwells with adhesive substrates and their further retrieval in a temperature dependent manner by exploiting the stimuli responsiveness of these microwells. The responsive polymer structure of the arrays can be used to thermally regulate the microwell diameters causing a mechanical force on the aggregates, subsequently facilitating the retrieval of cell aggregates from the microwells with high efficiency compared to PEG arrays. This approach can be potentially integrated into high-throughput systems and may become a versatile tool for various applications that require aggregate formation and experimentation, such as tissue engineering, drug discovery, and stem cell biology.
登录
查看更多内容
影响因子：
14
作者：
Hsiue, GH;Hsu, SH;Yang, IK
通讯作者：
Yang, IK
影响因子：
14
作者：
Fukuda, Junji;Khademhosseini, Ali;Langer, Robert
通讯作者：
Langer, Robert
影响因子：
3.7
作者：
Abu-Absi, SF;Friend, JR;Hu, WS
通讯作者：
Hu, WS
影响因子：
6.1
作者：
Kim, Dongshin;Beebe, David J.
通讯作者：
Beebe, David J.
影响因子：
--
作者：
Chia, SM;Leong, KW;Yu, H
通讯作者：
Yu, H