The bcl-2 knockout mouse exhibits marked changes in osteoblast phenotype and collagen deposition in bone as well as a mild growth plate phenotype
The bcl-2 knockout mouse exhibits marked changes in osteoblast phenotype and collagen deposition in bone as well as a mild growth plate phenotype
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DOI：
10.1046/j.1365-2613.1998.790411.x
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发表时间：
1998-10-01
影响因子：
3
通讯作者：
Wallis, GA
中科院分区：
文献类型：
--
作者：
Boot-Handford, RP;Michaelidis, TM;Wallis, GA
对 1 日龄 bcl-2 敲除小鼠和年龄匹配的对照小鼠的长骨进行组织学检查，发现骨长度、生长板结构或软骨内骨化阶段没有明显差异。在生长迟缓或“侏儒”的 35 天大的 bcl-2 敲除小鼠中，生长板的增殖区似乎比年龄匹配的野生型对照稍薄，并且第二骨化中心发育得不太好。 bcl-2 消融最显着的组织学影响是对成骨细胞和骨的影响。 35 天大的基因敲除小鼠骨骼中的成骨细胞数量远多于对照组，并且与正常的扁平细胞外观相比，成骨细胞具有立方体表型。此外，与对照组织相比，bcl-2 敲除小鼠骨中成骨细胞沉积的胶原蛋白是无序的，并且具有假编织外观。结果表明 Bcl-2 在控制体内成骨细胞表型和骨沉积方面发挥着重要作用。
Histological examination of long bones from 1-day-old bcl-2 knockout and age-matched control mice revealed no obvious differences in length of bone, growth plate architecture or stage of endochondral ossification. In 35-day-old bcl-2 knockout mice that are growth retarded or 'dwarfed', the proliferative zone of the growth plate appeared slightly thinner and the secondary centres of ossification less well developed than their age-matched wild-type controls. The most marked histological effects of bcl-2 ablation were on osteoblasts and bone. 35-day-old knockout mouse bones exhibited far greater numbers of osteoblasts than controls and the osteoblasts had a cuboidal phenotype in comparison with the normal flattened cell appearance. In addition, the collagen deposited by the osteoblasts in the bcl-2 knockout mouse bone was disorganized in comparison with control tissue and had a pseudo-woven appearance. The results suggest an important role for Bcl-2 in controlling osteoblast phenotype and bone deposition in vivo.