A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture.
A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture.
复制标题
一种微型生物反应器系统，用于评估灌注培养中细胞与设计基质的相互作用。
DOI：
10.1002/term.510
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发表时间：
2012
影响因子：
3.3
通讯作者：
Sun T
中科院分区：
文献类型：
--
作者：
Sun T
在组织工程中，化学和地形学线索通常使用静态细胞培养物开发，但随后直接应用于三维（3D）和灌注下的组织培养物。由于人类细胞对培养环境的变化非常敏感，因此在将其应用于组织工程之前，必须评估灌注环境中任何此类线索的性能。因此，本研究的目的是通过解决灌注对3D支架内细胞培养的影响来弥合静态和灌注培养之间的差距。为此，我们开发了一种按比例缩小的生物反应器系统，可以在灌注条件下评估纳入我们之前开发的管状ε-聚己内酯支架中的各种化学和地形线索的有效性。两种示例性细胞类型的研究（a）与静态培养相比，3D支架中快速和牢固的细胞粘附对于灌注培养中的细胞存活至关重要;因此，静态培养的细胞接种程序可能不适用，因此，在将3D支架应用于组织培养之前，有必要在灌注条件下重新评估细胞在不同表面上的附着;（B）连续培养基灌注对细胞扩散和存活有不利影响，这可以通过间歇灌注来平衡;（c）微槽在灌注条件下仍然保持其对细胞排列的影响，而介质灌注对成纤维细胞排列显示出额外的影响，但对星形胶质细胞在凹槽基底上的排列没有影响。这项研究表明，微型生物反应器系统通过弥合静态培养和灌注培养之间的差距，对于开发具有合适化学和地形线索的功能支架至关重要。版权所有© 2011约翰威利父子有限公司.
In tissue engineering, chemical and topographical cues are normally developed using static cell cultures but then applied directly to tissue cultures in three dimensions (3D) and under perfusion. As human cells are very sensitive to changes in the culture environment, it is essential to evaluate the performance of any such cues in a perfused environment before they are applied to tissue engineering. Thus, the aim of this research was to bridge the gap between static and perfusion cultures by addressing the effect of perfusion on cell cultures within 3D scaffolds. For this we developed a scaled‐down bioreactor system, which allows evaluation of the effectiveness of various chemical and topographical cues incorporated into our previously developed tubularε‐polycaprolactone scaffold under perfused conditions. Investigation of two exemplary cell types (fibroblasts and cortical astrocytes) using the miniaturized bioreactor indicated that: (a) quick and firm cell adhesion in the 3D scaffold was critical for cell survival in perfusion culture compared with static culture; thus, cell‐seeding procedures for static cultures might not be applicable, therefore it was necessary to re‐evaluate cell attachment on different surfaces under perfused conditions before a 3D scaffold was applied for tissue cultures; (b) continuous medium perfusion adversely influenced cell spread and survival, which could be balanced by intermittent perfusion; (c) micro‐grooves still maintained their influences on cell alignment under perfused conditions, while medium perfusion demonstrated additional influence on fibroblast alignment but not on astrocyte alignment on grooved substrates. This research demonstrated that the mini‐bioreactor system is crucial for the development of functional scaffolds with suitable chemical and topographical cues by bridging the gap between static culture and perfusion culture. Copyright © 2011 John Wiley & Sons, Ltd.