Lead sulfide nanoparticles increase cell wall chitin content and induce apoptosis in Saccharomyces cerevisiae.
Lead sulfide nanoparticles increase cell wall chitin content and induce apoptosis in Saccharomyces cerevisiae.
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DOI：
10.1016/j.jhazmat.2014.03.008
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发表时间：
2014-05
影响因子：
13.6
通讯作者：
Meiqing Sun;Qilin Yu;Mengyuan Hu;Zhenwei Hao;Chengdong Zhang;Mingchun Li
中科院分区：
文献类型：
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作者：
Meiqing Sun;Qilin Yu;Mengyuan Hu;Zhenwei Hao;Chengdong Zhang;Mingchun Li
虽然已经有许多关于细菌毒性的研究，但纳米颗粒对真菌的细胞毒性仍然知之甚少。我们研究了不同粒径的硫化铅颗粒对重要的模式真菌酿酒酵母的毒性。最小的颗粒产生最高的毒性，抑制细胞生长并降低细胞活力，可能反映了沉降减少和持续的细胞壁攻击。S.结果表明，细胞壁几丁质含量增加，细胞壁完整性信号通路的两个基因FKS 2和PRM5过表达。细胞壁应激增加了细胞内活性氧的浓度，导致线粒体功能障碍和细胞凋亡。溶解的铅离子对总毒性的贡献可以忽略不计。这些发现首次证明了真菌对纳米颗粒的生理保护反应，从而为评估金属纳米颗粒的环境影响提供了有用的信息。
Although there have been numerous studies on bacterial toxicity, the cytotoxicity of nanoparticles toward fungi remains poorly understood. We investigated the toxicity of various sizes of lead sulfide particles against the important model fungus,Saccharomyces cerevisiae. The smallest particle exerted the highest toxicity, inhibiting cell growth and decreasing cell viability, likely reflecting reduced sedimentation and persistent cell wall attack. In response to cell wall stress,S. cerevisiaeshowed an increase in the cell wall chitin content and the overexpression ofFKS2andPRM5, two genes of the cell wall integrity signaling pathway. Cell wall stress increased the concentration of intracellular reactive oxygen species, leading to mitochondrial dysfunction and cell apoptosis. The contribution of dissolved lead ions to the overall toxicity was negligible. These findings provide the first demonstration of the physiological protective response of a fungus toward nanoparticles, thereby contributing useful information to the assessment of the environmental impact of metal nanoparticles.