Membrane channel gene expression in human costal and articular chondrocytes.
Membrane channel gene expression in human costal and articular chondrocytes.
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人肋软骨细胞和关节软骨细胞中的膜通道基因表达。
DOI：
10.1080/15476278.2016.1181238
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发表时间：
2016-04-02
期刊：
影响因子：
2.3
通讯作者：
Stacey M
中科院分区：
文献类型：
--
作者：
Asmar A;Barrett-Jolley R;Werner A;Kelly R Jr;Stacey M
软骨细胞是所有类型软骨中发现的独特驻留细胞，其功能的关键是能够通过代谢活动的变化对机械负荷做出反应。这种机械转导特性部分是通过一系列表达的跨膜通道的活性介导的。离子通道、间隙连接蛋白和孔蛋白。离子通道的适当表达已被证明对于细胞外基质的产生至关重要，跨膜通道的差异表达与骨关节炎和阿尔伯斯-舍恩伯格等肌肉骨骼疾病相关。在这项研究中，我们分析了人类关节软骨和肋软骨（青少年和胎儿起源）软骨细胞通道组之间基因表达的一致性。值得注意的是，我们发现 14 个离子通道基因在关节软骨细胞和两种类型的肋软骨软骨细胞之间普遍表达。还有其他几种离子通道基因仅在关节（6 个基因）或肋软骨细胞（5 个基因）中表达。在胎儿和青少年肋软骨中观察到 BEST1 和 KCNJ2 (Kir2.1) 表达的显着差异。有趣的是，大 Ca2+ 激活钾通道（BKα 或 KCNMA1）在所有检查的软骨细胞中都高度表达。在所有软骨样本的软骨细胞中也观察到 Panx1、GJA1 (Cx43) 和 GJC1 (Cx45) 间隙连接基因的表达。总之，这些数据突出了来自不同解剖部位的细胞中软骨细胞膜通道基因表达之间的相似性，并且可能暗示共同的电生理信号传导途径是细胞控制的基础。一系列机械和代谢敏感膜通道的高表达表明软骨细胞机械转导可能比之前想象的更复杂。
Chondrocytes are the uniquely resident cells found in all types of cartilage and key to their function is the ability to respond to mechanical loads with changes of metabolic activity. This mechanotransduction property is, in part, mediated through the activity of a range of expressed transmembrane channels; ion channels, gap junction proteins, and porins. Appropriate expression of ion channels has been shown essential for production of extracellular matrix and differential expression of transmembrane channels is correlated to musculoskeletal diseases such as osteoarthritis and Albers-Schönberg. In this study we analyzed the consistency of gene expression between channelomes of chondrocytes from human articular and costal (teenage and fetal origin) cartilages. Notably, we found 14 ion channel genes commonly expressed between articular and both types of costal cartilage chondrocytes. There were several other ion channel genes expressed only in articular (6 genes) or costal chondrocytes (5 genes). Significant differences in expression of BEST1 and KCNJ2 (Kir2.1) were observed between fetal and teenage costal cartilage. Interestingly, the large Ca2+ activated potassium channel (BKα, or KCNMA1) was very highly expressed in all chondrocytes examined. Expression of the gap junction genes for Panx1, GJA1 (Cx43) and GJC1 (Cx45) was also observed in chondrocytes from all cartilage samples. Together, this data highlights similarities between chondrocyte membrane channel gene expressions in cells derived from different anatomical sites, and may imply that common electrophysiological signaling pathways underlie cellular control. The high expression of a range of mechanically and metabolically sensitive membrane channels suggest that chondrocyte mechanotransduction may be more complex than previously thought.