Induction of the acrosome reaction in sea urchin spermatozoa by the volatile anesthetic halothane.
Induction of the acrosome reaction in sea urchin spermatozoa by the volatile anesthetic halothane.
复制标题
挥发性麻醉剂氟烷诱导海胆精子顶体反应。
DOI：
10.1095/biolreprod34.1.119
复制
发表时间：
1986
影响因子：
3.6
通讯作者：
Greenberg,CA
中科院分区：
文献类型：
--
作者：
Hinkley,RE;Wright,BD;Greenberg,CA
挥发性麻醉剂氟烷快速、剂量依赖性地诱导海胆精子顶体反应。该反应在含10mMCa~(2+)的人工海水和低(<；100μM)-Ca~(2+)介质中发生得同样好。麻醉诱导的顶体反应可以通过用EGTA、镧或普鲁卡因预处理精子来预防。相反，钙通道阻滞剂D-600、维拉帕米、地尔硫卓和尼群二烯不能阻止这一反应。氟烷诱导的顶体突起在超微结构上与卵冻(顶体反应的天然诱导剂)诱导的顶体突起相同。这些结果表明，麻醉剂诱导的顶体反应需要少量的钙离子，并可能来自内源。安氟醚、异氟醚和甲氧基氟烷也会引起顶体反应，这表明挥发性麻醉剂可能有助于研究伴随精子激活而产生的离子变化。
The volatile anesthetic halothane rapidly and dose-dependently induces the acrosome reaction in sperm of the sea urchin Lytechinus variegatus. The reaction occurs equally well in artificial sea water containing 10 mM Ca2+and in low (<100 μM)-Ca2+media. The anesthetic-induced acrosome reaction can be prevented by pretreating sperm with EGTA, lanthanum, or procaine. In contrast, the Ca2+channel blockers D-600, verapamil, diltiazem, and nitrendipene do not prevent the reaction. Acrosomal processes induced by halothane are ultrastructurally identical to those induced by egg jelly, the natural inducer of the acrosome reaction. These results suggest that Ca2+in low quantity is required for the anesthetic-induced acrosome reaction and may be derived from internal sources. Enflurane, isoflurane, and methoxyflurane also induce the acrosome reaction, indicating that volatile anesthetics may be useful in studying ion changes accompanying sperm activation.