Advantage of Alveolar Ridge Augmentation with Bioactive/Bioresorbable Screws Made of Composites of Unsintered Hydroxyapatite and Poly-L-lactide
Advantage of Alveolar Ridge Augmentation with Bioactive/Bioresorbable Screws Made of Composites of Unsintered Hydroxyapatite and Poly-L-lactide
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DOI：
10.3390/ma12223681
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发表时间：
2019-11-02
期刊：
影响因子：
3.4
通讯作者：
Furuki, Yoshihiko
中科院分区：
文献类型：
--
作者：
Sukegawa, Shintaro;Kawai, Hotaka;Furuki, Yoshihiko
我们研究了人类骨愈合的特点和组织学成骨环境，通过使用由未煅烧和未烧结的羟基磷灰石（u-HA）和聚-L-乳酸（PLLA）的复合材料制成的装置。在8例固定病例中，我们使用u-HA/PLLA螺钉进行上颌牙槽嵴增高，在种植前手术中使用下颌骨皮质骨块。对5个适当的螺钉样本进行组织学和免疫组化评价，以检测runt相关转录因子2（RUNX 2）、转录因子Sp 7（Osterix）和瘦素受体（LepR）。在所有病例中，组织学评价显示骨成分完全包围u-HA/PLLA螺钉，骨直接连接到生物材料。炎症细胞没有侵入骨和u-HA/PLLA螺钉之间的空间。免疫组织化学评价显示，在u-HA/PLLA周围的组织中，许多细胞对RUNX 2或Osterix呈阳性，RUNX 2或Osterix是成骨细胞和骨祖细胞的标志物。此外，许多骨髓来源的间充质干细胞对LepR和RUNX 2均呈显著阳性。u-HA/PLLA复合材料具有良好的生物活性和生物相容性。此外，我们的研究结果表明，许多骨髓来源的间充质干细胞和成熟的成骨细胞存在于用u-HA/PLLA螺钉创建的成骨环境中，并且这种环境适合于成骨。
We studied human bone healing characteristics and the histological osteogenic environment by using devices made of a composite of uncalcined and unsintered hydroxyapatite (u-HA) and poly-L-lactide (PLLA). In eight cases of fixation, we used u-HA/PLLA screws for maxillary alveolar ridge augmentation, for which mandibular cortical bone block was used in preimplantation surgery. Five appropriate samples with screws were evaluated histologically and immunohistochemically for runt-related transcription factor 2 (RUNX2), transcription factor Sp7 (Osterix), and leptin receptor (LepR). In all cases, histological evaluation revealed that bone components had completely surrounded the u-HA/PLLA screws, and the bone was connected directly to the biomaterial. Inflammatory cells did not invade the space between the bone and the u-HA/PLLA screw. Immunohistochemical evaluation revealed that many cells were positive for RUNX2 or Osterix, which are markers for osteoblast and osteoprogenitor cells, in the tissues surrounding u-HA/PLLA. In addition, many bone marrow-derived mesenchymal stem cells were notably positive for both LepR and RUNX2. The u-HA/PLLA material showed excellent bioactive osteoconductivity and a highly biocompatibility with bone directly attached. In addition, our findings suggest that many bone marrow-derived mesenchymal stem cells and mature osteoblast are present in the osteogenic environment created with u-HA/PLLA screws and that this environment is suitable for osteogenesis.