Interactions among pulmonary surfactant, vernix caseosa, and intestinal enterocytes: intra-amniotic administration of fluorescently liposomes to pregnant rabbits
Interactions among pulmonary surfactant, vernix caseosa, and intestinal enterocytes: intra-amniotic administration of fluorescently liposomes to pregnant rabbits
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肺表面活性物质、胎脂和肠上皮细胞之间的相互作用：怀孕兔羊膜内注射荧光脂质体
DOI：
10.1152/ajplung.00081.2011
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Kotsuji F.
中科院分区：
文献类型：
--
作者：
Nishijima K;Shukunami K;Yoshinari H;Takahashi J;Maeda H;Takagi H;Kotsuji F.
虽然已知胎脂在出生时是天然生物膜，但人肺表面活性剂在妊娠34周时开始将胎脂从胎儿皮肤中移除到羊水中，即，在分娩前。为了解释这一矛盾，我们首先产生了两种类型的荧光标记的脂质体显示类似的肺表面活性物质和胎脂复合物的形态。然后我们将这些脂质体连续给药到妊娠兔的羊水空间中。此外，我们还制备了肺表面活性物质和胎脂复合物，并将其注入妊娠家兔的羊水腔。羊膜内灌注的荧光标记脂质体被吸收到胎儿肠上皮中。然而，脂质体不转运到胎兔的肝脏。我们还发现，连续给药的胶束来自肺表面活性剂和胎脂保护兔胎儿小肠损伤由于手术干预。我们的研究结果表明，肺表面活性物质和胎脂干酪复合物吞咽羊水可能局部影响胎儿小肠上皮细胞。虽然目前的研究主要是观察性的，需要进一步的研究，我们的研究结果阐明了肺，真皮表皮和胃肠道发育过程之间的生理相互作用。
Although vernix caseosa is known to be a natural biofilm at birth, human pulmonary surfactant commences to remove the vernix from fetal skin into the amniotic fluid atgestational week 34, i.e., well before delivery. To explain this paradox, we first produced two types of fluorescently labeled liposomes displaying morphology similar to that of pulmonary surfactant and vernix caseosa complexes. We then continuously administered these liposomes into the amniotic fluid space of pregnant rabbits. In addition, we produced pulmonary surfactant and vernix caseosa complexes and administered them into the amniotic fluid space of pregnant rabbits. The intra-amniotic infused fluorescently labeled liposomes were absorbed into the fetal intestinal epithelium. However, the liposomes were not transported to the livers of fetal rabbits. We also revealed that continuous administration of micelles derived from pulmonary surfactants and vernix caseosa protected the small intestine of the rabbit fetus from damage due to surgical intervention. Our results indicate that pulmonary surfactant and vernix caseosa complexes in swallowed amniotic fluid might locally influence fetal intestinal enterocytes. Although the present studies are primarily observational and further studies are needed, our findings elucidate the physiological interactions among pulmonary, dermal-epidermal, and gastrointestinal developmental processes.