Salinity and temperature affect the symbiont profile and host condition of Florida USA blue crabs Callinectes sapidus
Salinity and temperature affect the symbiont profile and host condition of Florida USA blue crabs Callinectes sapidus
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盐度和温度影响美国佛罗里达州蓝蟹 Callinectes sapidus 的共生体特征和寄主条件
DOI：
10.1016/j.jip.2023.107930
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发表时间：
2023
影响因子：
3.4
通讯作者：
Behringer, Donald C.
中科院分区：
文献类型：
--
作者：
Walters, Erin A.;Bojko, Jamie;Crowley, Claire E.;Gandy, Ryan L.;Martin, Charles W.;Shea, Colin P.;Bateman, Kelly S.;Stentiford, Grant D.;Behringer, Donald C.
亚热带佛罗里达蓝蟹，Callinectes sapidus，与温带蓝蟹相比，表现出不同的生活史特征，可能影响共生感染动态。关于佛罗里达的信息很少。sapidus共生特征、在不同生境中的分布及其对蟹类状况的影响。利用组织病理学，基因组学和透射电子显微镜，我们描述了佛罗里达州的第一个共生体概况。占据淡水到海洋栖息地的松毛虫。从409只螃蟹中鉴定出12个共生体类群，包括纤毛虫、地沟虫、微孢子虫、单孢子虫和血球虫。线虫，丝状细菌，绿格林菌，糙皮病菌，八爪虫，Cambarincolasp。，以及假定的微细胞。总的来说，78%的c。在野生种群中，有一个或多个共生体群显示出较高的感染率。环境变量水温和盐度解释了佛罗里达州栖息地共生类群变化的48%，盐度与c呈正相关。sapidussymbiont多样性。这表明淡水c。与咸水种群相比，sapidus拥有较少的共生体，代表着更健康的个体。使用反射作用死亡率预测器（RAMP）检查螃蟹状况，以确定反射损伤是否与共生体流行有关。共生体与螃蟹状况呈正相关，受损的螃蟹更有可能寄主共生体，这表明共生体包含可以提高RAMP应用的预测能力。微孢子虫共生体组对c的影响特别强。与其他共生体组相比，sapidus反射反应和损伤平均高1.57倍。我们的研究结果表明，考虑完整的共生体概况及其与空间和时间变化的环境的关联对于全面评估c的重要性。sapiduspopulation健康。
Subtropical Florida blue crabs,Callinectes sapidus, exhibit differing life history traits compared to their temperate counterparts, likely influencing symbiont infection dynamics. Little information exists for FloridaC. sapidussymbiont profiles, their distribution among various habitats, and influence on crab condition. Using histopathology, genomics, and transmission electron microscopy, we describe the first symbiont profiles for FloridaC. sapidusoccupying freshwater to marine habitats. Twelve symbiont groups were identified from 409 crabs including ciliophorans, digenean, microsporidian, Haplosporidia,Hematodiniumsp., Nematoda, filamentous bacteria, gregarine, Callinectes sapidus nudivirus,Octolasmissp.,Cambarincolasp., and putative microcell. Overall, 78% ofC. sapiduswere documented with one or more symbiont groups demonstrating high infection rates in wild populations. Environmental variables water temperature and salinity explained 48% of the variation in symbiont groups among Florida habitats, and salinity was positively correlated withC. sapidussymbiont diversity. This suggests freshwaterC. sapiduspossess fewer symbionts and represent healthier individuals compared to saltwater populations. Crab condition was examined using the reflex action mortality predictor (RAMP) to determine if reflex impairment could be linked to symbiont prevalence. Symbionts were found positively correlated with crab condition, and impaired crabs were more likely to host symbionts, demonstrating symbiont inclusion may boost predictive ability of the RAMP application. The microsporidian symbiont group had a particularly strong effect onC. sapidusreflex response, and impairment was on average 1.57 times higher compared to all other symbiont groups. Our findings demonstrate the importance of considering full symbiont profiles and their associations with a spatially and temporally variable environment to fully assessC. sapiduspopulation health.