Inhibition of E. coli DNA polymerase I by 1,10-phenanthroline.
Inhibition of E. coli DNA polymerase I by 1,10-phenanthroline.
复制标题
1,10-菲咯啉对大肠杆菌 DNA 聚合酶 I 的抑制作用。
DOI：
10.1016/0006-291x(77)91236-0
复制
发表时间：
1977
影响因子：
3.1
通讯作者：
D. Sigman
中科院分区：
文献类型：
--
作者：
Vito D'Aurora;A. Stern;D. Sigman
Abstract A 1, 10-phenanthroline-cuprous ion complex is a potent reversible inhibitor of E. coli DNA polymerase I yielding 50% inhibition in the micromolar concentration range. The 2: 1 1, 10-phenanthroline-cuprous ion complex is most probably the inhibitory species. Complexes of cupric ion and 1, 10-phenanthroline have no apparent kinetic effect. The previously reported inhibition of the enzyme by 1, 10-phenanthroline (1, 2) is most likely due to the formation of this complex from thiols normally added to the assay mixtures and trace amounts of cupric ion invariably present notwithstanding reasonable precaution. The reversible and instantaneous 1, 10-phenanthroline inhibition observed for other polymerases may be due to this unique inhibitory species and not coordination of a catalytically important zinc ion at the active site by the chelating agent.
