Variable expression levels detected in the Drosophila effectors of piRNA biogenesis
Variable expression levels detected in the Drosophila effectors of piRNA biogenesis
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DOI：
10.1016/j.gene.2013.11.095
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发表时间：
2014-03-01
期刊：
影响因子：
3.5
通讯作者：
Vieira, Cristina
中科院分区：
文献类型：
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作者：
Fablet, Marie;Akkouche, Abdou;Vieira, Cristina
piRNA（piwi-interacting RNAs）是一类在果蝇转座因子（transposable elements，TE）调控中起重要作用的小分子干扰RNA，被认为在性腺发育中具有重要意义。因此，编码皮尔纳机制的效应子的基因通常被认为是高度受限的。相反，作为遗传免疫的参与者，这些基因也被证明进化迅速，并显示出高水平的序列变异性。为了评估对这些竞争模型的支持，我们使用一组野生型模拟果蝇菌株分析了皮尔纳途径的7个基因，已知这些菌株之间的TE含量存在显著差异。我们发现，这些基因表现出广泛的转录水平的变化，我们讨论了一些进化方面的考虑，所观察到的变异TE拷贝数。(C)2014爱思唯尔有限公司版权所有。
piRNAs (piwi-interacting RNAs) are a class of small interfering RNAs that play a major role in the regulation of transposable elements (TEs) in Drosophila and are considered of fundamental importance in gonadal development. Genes encoding the effectors of the piRNA machinery are thus often thought to be highly constrained. On the contrary, as actors of genetic immunity, these genes have also been shown to evolve rapidly and display a high level of sequence variability. In order to assess the support for these competing models, we analyzed seven genes of the piRNA pathway using a collection of wild-type strains of Drosophila simulans, which are known to display significant variability in their TE content between strains. We showed that these genes exhibited wide variation in transcript levels, and we discuss some evolutionary considerations regarding the observed variability in TE copy numbers. (C) 2014 Elsevier B.V. All rights reserved.