Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles
Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles
复制标题
利用金纳米颗粒表面等离子共振光散射直接测定尿液溶菌酶
DOI：
10.1016/j.talanta.2010.05.034
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发表时间：
2010-07-15
期刊：
影响因子：
6.1
通讯作者：
Li, Na
中科院分区：
文献类型：
--
作者：
Wang, Xinyi;Xu, Yao;Li, Na
本研究以纳米金为探针，建立了一种简便、灵敏的溶菌酶分析方法--等离子体共振光散射技术。纳摩尔水平的溶菌酶通过增强PRLS诱导AuNPs聚集。对于直径为13 nm的1.4 nm柠檬酸盐包覆的AuNPs，其线性范围为15-50 nm，对溶菌酶的检出限为13.1 nm。六个纳米分子溶菌酶可以产生明显的PRLS增强。尿液中存在的大多数潜在干扰物质对测定的影响可以忽略不计。尿样中人血清白蛋白的干扰可用pH 4.8-4.9的乙醇沉淀来减少。对8份单份添加溶菌酶的尿样的回收率为90.1-118.2%。该方法简便、灵敏，不需要样品净化和AuNPs修饰，为尿溶菌酶的检测提供了一种新的方法。(C)2010爱思唯尔B.V.保留所有权利。
The purpose of this study was to establish a simple and sensitive analytical method for lysozyme using Plasmon Resonance Light-Scattering (PRLS) technique with Gold Nanoparticles (AuNPs) as the probe. Nanomolar level of lysozyme induced AuNPs aggregation with enhanced PRLS. For 1.4 nM citrate-capped AuNPs (13 nm in diameter), the linear range of the calibration curve was 15-50 nM with a detection limit of 13.1 nM for lysozyme. Six nanomolar lysozyme can produce an observable PRLS enhancement. Most potential interfering substances present in urine had a negligible effect on the determination. The interference from human serum albumin in the urinary sample can be reduced by precipitating the albumin with ethanol at pH 4.8-4.9. The 90.1-118.2% recovery was achieved for 8 individual lysozyme-spiked urinary samples. This simple and sensitive method for lysozyme does not require sample clean-up and AuNPs modification, thus provided an alternative for urinary lysozyme determination. (C) 2010 Elsevier B.V. All rights reserved.