Fatness QTL on chicken chromosome 5 and interaction with sex
Fatness QTL on chicken chromosome 5 and interaction with sex
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DOI：
10.1051/gse:2006005
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发表时间：
2006-05-01
影响因子：
4.1
通讯作者：
Douaire, M
中科院分区：
文献类型：
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作者：
Abasht, B;Pittel, F;Douaire, M
先前在三代设计中在 5 号染色体 (GGA5) 上鉴定了影响雄性鸡肥胖的数量性状位点 (QTL)，该三代设计源自根据腹部脂肪重量不同选择的两个实验鸡品系。基于相同的纯系建立的新设计，从 4 个 F1 父系家族中产生了 407 个 F2 后代（雄性和雌性）。在 9 周龄时测量 F2 的体重和腹部脂肪。在每个父系家系中，使用 GGA5 的 7 个微卫星标记对 48 个极端个体的腹部脂肪进行了选择性基因分型。 QTL 分析证实了 GGA5 上肥胖 QTL 的存在，并通过性别相互作用鉴定了 QTL。通过将 QTL 杂合的 F1 父本与瘦系母本杂交，产生了三个重组回交 1 (BC1) 雄性，并在回交 2 (BC2) 后代中评估了它们的 QTL 基因型。这些结果通过 F2 代中鉴定的性别相互作用证实了 QTL，并且它们允许将雌性 QTL 定位到 GGA5 远端部分小于 8 Mb。他们还表明，脂肪 QTL 等位基因在脂肪系和瘦肉系中均发生分离。
Quantitative trait loci (QTL) affecting fatness in male chickens were previously identified on chromosome 5 (GGA5) in a three-generation design derived from two experimental chicken lines divergently selected for abdominal fat weight. A new design, established from the same pure lines, produced 407 F2 progenies (males and females) from 4 F1-sire families. Body weight and abdominal fat were measured on the F2 at 9 wk of age. In each sire family, selective genotyping was carried out for 48 extreme individuals for abdominal fat using seven microsatellite markers from GGA5. QTL analyses confirmed the presence of QTL for fatness on GGA5 and identified a QTL by sex interaction. By crossing one F1 sire heterozygous at the QTL with lean line dams, three recombinant backcross 1 (BC1) males were produced and their QTL genotypes were assessed in backcross 2 (BC2) progenies. These results confirmed the QTL by sex interaction identified in the F2 generation and they allow mapping of the female QTL to less than 8 Mb at the distal part of the GGA5. They also indicate that fat QTL alleles were segregating in both fat and lean lines.