The effect of lead on calcium release activated calcium influx in primary cultures of human osteoblast-like cells
The effect of lead on calcium release activated calcium influx in primary cultures of human osteoblast-like cells
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DOI：
10.1007/s12272-001-1140-3
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发表时间：
2008-02-01
影响因子：
6.7
通讯作者：
Yun, Il
中科院分区：
文献类型：
--
作者：
Jang, Hye-Ock;Kim, Ji-Suk;Yun, Il
为了进一步了解骨骼作为铅毒性靶组织以及全身铅储存库的重要性，有必要确定铅对人成骨细胞样细胞（OLC）原代培养物中钙释放激活的钙内流（CRACI）的影响。 Pb2+以剂量依赖性方式立即抑制CRACI。 Pb2+ 流入人 OLC 的量呈剂量依赖性增加。本研究表明，Pb2+ 对人类 OLC 的 CRACI 的干扰至少是双重的：(1) CRACI 的启动，即 Ca2+ 再添加时可测量的 Ca2+ 流入，被 Pb2+ 部分抑制；(2) Pb2+ 的流入在 CRACI 诱导后增强。
To further understand the significance of bone as a target tissues of lead toxicity, as well as a reservoir of systemic lead, it is necessary to define the effect of lead on the calcium release activated calcium influx (CRACI) in primary cultures of human osteoblast-like cells (OLC). Pb2+ inhibited the immediate CRACI dose-dependent manner. Influx of Pb2+ into human OLC was increased dose-dependent manner. The present study demonstrates that the interference of Pb2+ with CRACI of human OLC is at least twofold: (1) the initiation of CRACI, i.e., the measurable influx of Ca2+ upon Ca2+ readdition, is partially inhibited by Pb2+ and (2) the influx of Pb2+, was enhanced after CRACI had been induced.