Possible roles for microtubules and microfilaments in ADH action on toad urinary bladder.
Possible roles for microtubules and microfilaments in ADH action on toad urinary bladder.
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微管和微丝在 ADH 对蟾蜍膀胱的作用中可能发挥的作用。
DOI：
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发表时间：
1979
影响因子：
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通讯作者：
J. Muller
中科院分区：
文献类型：
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作者：
W. Kachadorian;S. J. Ellis;J. Muller
已发现加压素刺激后蟾蜍膀胱颗粒细胞的管腔膜中的膜内颗粒聚集体与水渗透性的诱导改变密切相关。微管和微丝在介导后一种反应中的作用已分别基于涉及秋水仙素和细胞松弛素B的研究而提出。在本研究中，这些药物对加压素诱导的渗透水流的启动和维持以及颗粒聚集现象的影响进行了研究。结果表明，在启动过程中的聚集和水流量的反应，加压素是每个秋水仙素和细胞松弛素B敏感，这些敏感性可以完全加。然而，在完全加压素刺激建立后，相同的反应仅表现出对细胞松弛素B的敏感性，而对秋水仙碱不敏感。因此，研究结果表明，微管和微丝可能是独立必要的启动的聚集和水流反应加压素，和微丝，而不是微管，需要它们的维护。
Intramembranous particle aggregates in the luminal membrane of toad bladder granular cells after vasopressin stimulation have been found to correlate closely and specifically with induced alterations of water permeability. Roles for microtubules and microfilaments in mediating the latter response have been proposed on the basis of studies involving colchicine and cytochalasin B, respectively. In the present investigation the effects of these agents on both initiating and sustaining vasopressin-induced osmotic water flow and the particle aggregation phenomenon were studied. The results indicate that during initiation the aggregation and water flow responses to vasopressin are each colchicine- and cytochalasin B-sensitive and that these sensitivities can be wholly additive. However, after full vasopressin stimulation is established, the same responses demonstrate sensitivity only to cytochalasin B, not to colchicine. The findings, therefore, suggest that microtubules and microfilaments may be independently necessary for the initiation of the aggregation and water flow responses to vasopressin, and that microfilaments, but not microtubules, are required for their maintenance.