Transposon Calling Cards.
Transposon Calling Cards.
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DOI：
10.1101/pdb.top077776
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发表时间：
2016-02-01
影响因子：
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通讯作者：
Mitra, Robi D
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文献类型：
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作者：
Mayhew, David;Mitra, Robi D
Identifying the genomic targets of transcription factors is an important step in understanding the regulatory networks of gene transcription in yeast. We have developed a method that utilizes what we refer to as transposon "calling cards," in which a transcription factor directs the Ty5 retrotransposase to insert transposons into the genome adjacent to where the transcription factor binds. This method is designed to be multiplexed with many barcoded transcription factors and has the potential to decrease the labor required for the study of large numbers of transcription factors.
